%0 Journal Article %1 Huang2016 %A Huang, Fang %A Sirinakis, George %A Allgeyer, Edward S. %A Schroeder, Lena K. %A Duim, Whitney C. %A Kromann, Emil B. %A Phan, Thomy %A Rivera-Molina, Felix E. %A Myers, Jordan R. %A Irnov, Irnov %A Lessard, Mark %A Zhang, Yongdeng %A Handel, Mary Ann %A Jacobs-Wagner, Christine %A Lusk, C. Patrick %A Rothman, James E. %A Toomre, Derek %A Booth, Martin J. %A Bewersdorf, Joerg %D 2016 %I The Authors %J Cell %K 3D fluorescence microscopy superresolution %N 4 %P 1028--1040 %R 10.1016/j.cell.2016.06.016 %T Ultra-High Resolution 3D Imaging of Whole Cells %U http://dx.doi.org/10.1016/j.cell.2016.06.016 %V 166 %X Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in cell biological research. However, because of its usually inferior resolution in the depth direction (50–80 nm) and rapidly deteriorating resolution in thick samples, its practical biological application has been effectively limited to two dimensions and thin samples. Here, we present the development of whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), an optical nanoscope that allows imaging of three-dimensional (3D) structures at 10- to 20-nm resolution throughout entire mammalian cells. We demonstrate the wide applicability of W-4PiSMSN across diverse research fields by imaging complex molecular architectures ranging from bacteriophages to nuclear pores, cilia, and synaptonemal complexes in large 3D cellular volumes.

@article{Huang2016, abstract = {Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in cell biological research. However, because of its usually inferior resolution in the depth direction (50–80 nm) and rapidly deteriorating resolution in thick samples, its practical biological application has been effectively limited to two dimensions and thin samples. Here, we present the development of whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), an optical nanoscope that allows imaging of three-dimensional (3D) structures at 10- to 20-nm resolution throughout entire mammalian cells. We demonstrate the wide applicability of W-4PiSMSN across diverse research fields by imaging complex molecular architectures ranging from bacteriophages to nuclear pores, cilia, and synaptonemal complexes in large 3D cellular volumes.}, added-at = {2020-03-23T21:12:34.000+0100}, author = {Huang, Fang and Sirinakis, George and Allgeyer, Edward S. and Schroeder, Lena K. and Duim, Whitney C. and Kromann, Emil B. and Phan, Thomy and Rivera-Molina, Felix E. and Myers, Jordan R. and Irnov, Irnov and Lessard, Mark and Zhang, Yongdeng and Handel, Mary Ann and Jacobs-Wagner, Christine and Lusk, C. Patrick and Rothman, James E. and Toomre, Derek and Booth, Martin J. and Bewersdorf, Joerg}, biburl = {https://www.bibsonomy.org/bibtex/26f089864f3cc202eec7440cee130b807/kfriedl}, doi = {10.1016/j.cell.2016.06.016}, file = {:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Huang et al. - 2016 - Ultra-High Resolution 3D Imaging of Whole Cells.pdf:pdf}, interhash = {77945cf783f9a4f10851930d990d662c}, intrahash = {6f089864f3cc202eec7440cee130b807}, issn = {10974172}, journal = {Cell}, keywords = {3D fluorescence microscopy superresolution}, month = aug, number = 4, pages = {1028--1040}, publisher = {The Authors}, timestamp = {2020-03-23T21:54:09.000+0100}, title = {{Ultra-High Resolution 3D Imaging of Whole Cells}}, url = {http://dx.doi.org/10.1016/j.cell.2016.06.016}, volume = 166, year = 2016 }