%0 Journal Article %1 xu2017microrna21 %A Xu, Bin %A Xia, Hehuan %A Cao, Junming %A Wang, Zhihong %A Yang, Yipeng %A Lin, Yongsheng %D 2017 %J Oncology Research %K 8 cancer epigenetics methods mirnas %N 7 %P 1161-1168 %R 10.3727/096504017X14841698396829 %T MicroRNA-21 Inhibits the Apoptosis of Osteosarcoma Cell Line SAOS-2 via Targeting Caspase 8 %U http://www.ingentaconnect.com/content/cog/or/2017/00000025/00000007/art00013 %V 25 %X Currently, multiple microRNAs (miRNAs) have been found to play vital roles in the pathogenesis of osteosarcoma. This study aimed to investigate the role of miR-21 in osteosarcoma. The level of miR-21 in 20 pairs of osteosarcoma and corresponding adjacent tissues was monitored by qPCR. Human osteosarcoma cell line SAOS-2 was transfected with either miR-21 mimic or miR-21 inhibitor, and then cell viability, survival, and apoptosis were measured by MTT, colony formation assay, and flow cytometry. A target of miR-21 was predicted by the microRNA.org database and verified in vitro by using luciferase reporter, qPCR, and Western blot analyses. Finally, cells were cotransfected with siRNA against caspase 8 and miR-21 inhibitor, and the apoptotic cell rate was determined again. Results showed that the mRNA level of miR-21 was highly expressed in osteosarcoma tissues compared with adjacent tissues. Overexpression of miR-21 improved cell viability and survival but suppressed apoptosis. Caspase 8 was a direct target of miR-21, and it was negatively regulated by miR-21. Moreover, miR-21 suppression attenuated caspase 8 silencing and induced the decrease in apoptosis. In conclusion, overexpression of miR-21 suppressed SAOS-2 cell apoptosis via directly targeting caspase 8.

@article{xu2017microrna21, abstract = {Currently, multiple microRNAs (miRNAs) have been found to play vital roles in the pathogenesis of osteosarcoma. This study aimed to investigate the role of miR-21 in osteosarcoma. The level of miR-21 in 20 pairs of osteosarcoma and corresponding adjacent tissues was monitored by qPCR. Human osteosarcoma cell line SAOS-2 was transfected with either miR-21 mimic or miR-21 inhibitor, and then cell viability, survival, and apoptosis were measured by MTT, colony formation assay, and flow cytometry. A target of miR-21 was predicted by the microRNA.org database and verified in vitro by using luciferase reporter, qPCR, and Western blot analyses. Finally, cells were cotransfected with siRNA against caspase 8 and miR-21 inhibitor, and the apoptotic cell rate was determined again. Results showed that the mRNA level of miR-21 was highly expressed in osteosarcoma tissues compared with adjacent tissues. Overexpression of miR-21 improved cell viability and survival but suppressed apoptosis. Caspase 8 was a direct target of miR-21, and it was negatively regulated by miR-21. Moreover, miR-21 suppression attenuated caspase 8 silencing and induced the decrease in apoptosis. In conclusion, overexpression of miR-21 suppressed SAOS-2 cell apoptosis via directly targeting caspase 8.}, added-at = {2017-10-26T01:33:32.000+0200}, author = {Xu, Bin and Xia, Hehuan and Cao, Junming and Wang, Zhihong and Yang, Yipeng and Lin, Yongsheng}, biburl = {https://www.bibsonomy.org/bibtex/23401a80689859e4fd15157a7248bfda3/artheibault}, description = {This article describes a study of miR-21 regulation in osteosarcoma. The researchers detected upregulation of miR-21 and greater binding of miR-21 to caspase 8 in tumor tissue. To discern potential targets of miR-21 the researchers used microrna.org. Thus, the study illustrates that scientists continue to use microrna.org to seek out information on miRNA binding sites.}, doi = {10.3727/096504017X14841698396829}, interhash = {7dea850d8fbac5a442fb74df5a8a57c2}, intrahash = {3401a80689859e4fd15157a7248bfda3}, journal = {Oncology Research}, keywords = {8 cancer epigenetics methods mirnas}, number = 7, pages = {1161-1168}, timestamp = {2017-10-26T01:33:32.000+0200}, title = {MicroRNA-21 Inhibits the Apoptosis of Osteosarcoma Cell Line SAOS-2 via Targeting Caspase 8}, url = {http://www.ingentaconnect.com/content/cog/or/2017/00000025/00000007/art00013}, volume = 25, year = 2017 }