%0 Journal Article %1 borrow_siad_1998 %A Borrow, R %A Claus, H %A Chaudhry, U %A Guiver, M %A Kaczmarski, E B %A Frosch, M %A Fox, A J %D 1998 %J FEMS Microbiology Letters %K , Assay, Bacterial, Chain Fragment Genes, Immunosorbent Infections, Length, Meningococcal Neisseria Polymerase Polymorphism, Reaction, Restriction Serotyping, Sialyltransferases bacterial_capsules meningitidis, {Enzyme-Linked} %N 2 %P 209--214 %T siaD PCR ELISA for confirmation and identification of serogroup Y and W135 meningococcal infections %U http://www.ncbi.nlm.nih.gov/pubmed/9503614 %V 159 %X Non-culture diagnosis and serogroup determination of meningococcal infection is important in contact management where vaccination may be possible. A serogroup B and C PCR ELISA assay for the non-culture diagnosis and serogroup determination has proved invaluable for enhanced epidemiological surveillance and contact management. A polymerase chain reaction assay, based on a restriction fragment length polymorphism in the meningococcal serogroup Y and W135 sialyltransferase (siaD) gene, was developed to enhance the range of non-culture diagnosis of meningococcal infection from clinical samples. The PCR assay was adapted to an ELISA format incorporating hybridisation with serogroup-specific Y and W135 oligonucleotide probes. The serogroup-specific W135 and Y PCR ELISA is a useful addition to currently available serogroup B and C assay for non-culture diagnosis of meningococcal infection and outbreak investigation.

@article{borrow_siad_1998, abstract = {Non-culture diagnosis and serogroup determination of meningococcal infection is important in contact management where vaccination may be possible. A serogroup B and C {PCR} {ELISA} assay for the non-culture diagnosis and serogroup determination has proved invaluable for enhanced epidemiological surveillance and contact management. A polymerase chain reaction assay, based on a restriction fragment length polymorphism in the meningococcal serogroup Y and W135 sialyltransferase {(siaD)} gene, was developed to enhance the range of non-culture diagnosis of meningococcal infection from clinical samples. The {PCR} assay was adapted to an {ELISA} format incorporating hybridisation with serogroup-specific Y and W135 oligonucleotide probes. The serogroup-specific W135 and Y {PCR} {ELISA} is a useful addition to currently available serogroup B and C assay for non-culture diagnosis of meningococcal infection and outbreak investigation.}, added-at = {2011-06-24T11:21:47.000+0200}, author = {Borrow, R and Claus, H and Chaudhry, U and Guiver, M and Kaczmarski, E B and Frosch, M and Fox, A J}, biburl = {https://www.bibsonomy.org/bibtex/218d1b5beea420ec878807f1420abbcfd/ag_vogel}, interhash = {82a3f7d17293d7b94461bae8e7cdfc1f}, intrahash = {18d1b5beea420ec878807f1420abbcfd}, issn = {0378-1097}, journal = {{FEMS} Microbiology Letters}, keywords = {, Assay, Bacterial, Chain Fragment Genes, Immunosorbent Infections, Length, Meningococcal Neisseria Polymerase Polymorphism, Reaction, Restriction Serotyping, Sialyltransferases bacterial_capsules meningitidis, {Enzyme-Linked}}, month = feb, note = {{PMID:} 9503614}, number = 2, pages = {209--214}, timestamp = {2011-08-08T15:47:34.000+0200}, title = {{siaD} {PCR} {ELISA} for confirmation and identification of serogroup Y and W135 meningococcal infections}, url = {http://www.ncbi.nlm.nih.gov/pubmed/9503614}, volume = 159, year = 1998 }