%0 Journal Article %1 citeulike:470438 %A Haga, Makoto %A Sugawara, Shinya %A Itagaki, Hiroshi %D 1981 %J Analytical Biochemistry %K l-i-l-a competition complement immunoassay homogeneous ag_detection liposome %N 2 %P 286--293 %R 10.1016/0003-2697(81)90584-4 %T Drug sensor: Liposome immunosensor for theophylline %U http://www.sciencedirect.com/science/article/B6W9V-4DXBSYR-57/2/f5081adb7addda3c72dc8fa1e02a4bbc %V 118 %X A new simple, sensitive liposome immunosensor has been developed by combining the advantages of spin membrane immunoassay and enzyme immunosensor. Theophylline, which is widely used in the treatment of acute and chronic respiratory diseases, was chosen as a model molecule. The liposome immunosensor system is composed of a Clark-type oxygen electrode and actively sensitized liposomes. It records liposome lysis induced by specific antitheophlline antibodies and complement which are monitored by the release of entrapped enzymes instead of spin labels. Sensitive detection was possible because of the double amplification of the antigen-antibody complexing reaction by the liposome lysis and enzymatic reaction. The sensitivity of liposome immunosensor was limited by the affinity of antibody rather than the electrode system. Theophylline was detectable down to a concentration of 0.7 ng/ml (4 x 10-9 ). Caffeine and theobromine cross-reacted slightly. The cross-reactivities of other xanthine derivatives were negligible. The liposome immunosensor offers a practical, simple, rapid, and sensitive method for the measurement of serum concentration of drugs.

@article{citeulike:470438, abstract = {A new simple, sensitive liposome immunosensor has been developed by combining the advantages of spin membrane immunoassay and enzyme immunosensor. Theophylline, which is widely used in the treatment of acute and chronic respiratory diseases, was chosen as a model molecule. The liposome immunosensor system is composed of a Clark-type oxygen electrode and actively sensitized liposomes. It records liposome lysis induced by specific antitheophlline antibodies and complement which are monitored by the release of entrapped enzymes instead of spin labels. Sensitive detection was possible because of the double amplification of the antigen-antibody complexing reaction by the liposome lysis and enzymatic reaction. The sensitivity of liposome immunosensor was limited by the affinity of antibody rather than the electrode system. Theophylline was detectable down to a concentration of 0.7 ng/ml (4 x 10-9 ). Caffeine and theobromine cross-reacted slightly. The cross-reactivities of other xanthine derivatives were negligible. The liposome immunosensor offers a practical, simple, rapid, and sensitive method for the measurement of serum concentration of drugs.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Haga, Makoto and Sugawara, Shinya and Itagaki, Hiroshi}, biburl = {https://www.bibsonomy.org/bibtex/2010145e6a6a1cf31e22ecdde81b6cfd1/biblio24}, citeulike-article-id = {470438}, doi = {10.1016/0003-2697(81)90584-4}, interhash = {858c2620d9e5b1a94eb9dbc1a2646b47}, intrahash = {010145e6a6a1cf31e22ecdde81b6cfd1}, journal = {Analytical Biochemistry}, keywords = {l-i-l-a competition complement immunoassay homogeneous ag_detection liposome}, month = {December}, number = 2, pages = {286--293}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Drug sensor: Liposome immunosensor for theophylline}, url = {http://www.sciencedirect.com/science/article/B6W9V-4DXBSYR-57/2/f5081adb7addda3c72dc8fa1e02a4bbc}, volume = 118, year = 1981 }