%0 Journal Article %1 vogel_necessity_1998 %A Vogel, U %A Morelli, G %A Zurth, K %A Claus, H %A Kriener, E %A Achtman, M %A Frosch, M %D 1998 %J Journal of Clinical Microbiology %K Adolescent, Bacterial, Carrier Chain Czech Female, Genes, Germany, Humans, Male, Meningitis, Meningococcal, Neisseria Parents, Polymerase Reaction, Republic, Serotyping State, meningitidis, %N 9 %P 2465--2470 %T Necessity of molecular techniques to distinguish between Neisseria meningitidis strains isolated from patients with meningococcal disease and from their healthy contacts %U http://www.ncbi.nlm.nih.gov/pubmed/9705375 %V 36 %X Serogroup C strains of Neisseria meningitidis were isolated from a Germany patient with severe meningococcal disease after a trip to the Czech Republic. These strains (case isolates) were characterized by classical and molecular techniques, as were other strains (carrier isolates) isolated from healthy contacts. Five of 10 carrier isolates had switched off the expression of capsular polysaccharide, as demonstrated by a serogroup-specific PCR. The two case isolates were indistinguishable by multilocus sequence typing and belonged to the ET-37 complex. The carrier isolates belonged to four different sequence types, all unrelated to that of the case strains. Pulsed-field gel electrophoresis showed that the case isolates differed from reference ET-37 complex strains from the Czech Republic and Canada as well as from all the carrier isolates. The isolate from the patient's nasopharynx was indistinguishable from the blood isolate except for a 40,000-bp chromosomal deletion that had occurred during systemic spread.

@article{vogel_necessity_1998, abstract = {Serogroup C strains of Neisseria meningitidis were isolated from a Germany patient with severe meningococcal disease after a trip to the Czech Republic. These strains (case isolates) were characterized by classical and molecular techniques, as were other strains (carrier isolates) isolated from healthy contacts. Five of 10 carrier isolates had switched off the expression of capsular polysaccharide, as demonstrated by a serogroup-specific {PCR.} The two case isolates were indistinguishable by multilocus sequence typing and belonged to the {ET-37} complex. The carrier isolates belonged to four different sequence types, all unrelated to that of the case strains. Pulsed-field gel electrophoresis showed that the case isolates differed from reference {ET-37} complex strains from the Czech Republic and Canada as well as from all the carrier isolates. The isolate from the patient's nasopharynx was indistinguishable from the blood isolate except for a 40,000-bp chromosomal deletion that had occurred during systemic spread.}, added-at = {2011-06-24T11:21:47.000+0200}, author = {Vogel, U and Morelli, G and Zurth, K and Claus, H and Kriener, E and Achtman, M and Frosch, M}, biburl = {https://www.bibsonomy.org/bibtex/224c282cea7405a7c2b71004fac242951/ag_vogel}, interhash = {8a8d9432a0d57f65957743e35485ba2a}, intrahash = {24c282cea7405a7c2b71004fac242951}, issn = {0095-1137}, journal = {Journal of Clinical Microbiology}, keywords = {Adolescent, Bacterial, Carrier Chain Czech Female, Genes, Germany, Humans, Male, Meningitis, Meningococcal, Neisseria Parents, Polymerase Reaction, Republic, Serotyping State, meningitidis,}, month = sep, note = {{PMID:} 9705375}, number = 9, pages = {2465--2470}, timestamp = {2011-06-24T11:21:54.000+0200}, title = {Necessity of molecular techniques to distinguish between Neisseria meningitidis strains isolated from patients with meningococcal disease and from their healthy contacts}, url = {http://www.ncbi.nlm.nih.gov/pubmed/9705375}, volume = 36, year = 1998 }