%0 Journal Article %1 dindas2021voltagedependent %A Dindas, J. %A Dreyer, I. %A Huang, S. %A Hedrich, R. %A Roelfsema, M. R. G. %D 2021 %J New Phytol %K *Arabidopsis/metabolism myOwn %N 4 %P 1449-1460 %R 10.1111/nph.17272 %T A voltage-dependent Ca(2+) homeostat operates in the plant vacuolar membrane %U https://www.ncbi.nlm.nih.gov/pubmed/33577135 %V 230 %X Cytosolic calcium signals are evoked by a large variety of biotic and abiotic stimuli and play an important role in cellular and long distance signalling in plants. While the function of the plasma membrane in cytosolic Ca(2+) signalling has been intensively studied, the role of the vacuolar membrane remains elusive. A newly developed vacuolar voltage clamp technique was used in combination with live-cell imaging, to study the role of the vacuolar membrane in Ca(2+) and pH homeostasis of bulging root hair cells of Arabidopsis. Depolarisation of the vacuolar membrane caused a rapid increase in the Ca(2+) concentration and alkalised the cytosol, while hyperpolarisation led to the opposite responses. The relationship between the vacuolar membrane potential, the cytosolic pH and Ca(2+) concentration suggests that a vacuolar H(+) /Ca(2+) exchange mechanism plays a central role in cytosolic Ca(2+) homeostasis. Mathematical modelling further suggests that the voltage-dependent vacuolar Ca(2+) homeostat could contribute to calcium signalling when coupled to a recently discovered K(+) channel-dependent module for electrical excitability of the vacuolar membrane.

@article{dindas2021voltagedependent, abstract = {Cytosolic calcium signals are evoked by a large variety of biotic and abiotic stimuli and play an important role in cellular and long distance signalling in plants. While the function of the plasma membrane in cytosolic Ca(2+) signalling has been intensively studied, the role of the vacuolar membrane remains elusive. A newly developed vacuolar voltage clamp technique was used in combination with live-cell imaging, to study the role of the vacuolar membrane in Ca(2+) and pH homeostasis of bulging root hair cells of Arabidopsis. Depolarisation of the vacuolar membrane caused a rapid increase in the Ca(2+) concentration and alkalised the cytosol, while hyperpolarisation led to the opposite responses. The relationship between the vacuolar membrane potential, the cytosolic pH and Ca(2+) concentration suggests that a vacuolar H(+) /Ca(2+) exchange mechanism plays a central role in cytosolic Ca(2+) homeostasis. Mathematical modelling further suggests that the voltage-dependent vacuolar Ca(2+) homeostat could contribute to calcium signalling when coupled to a recently discovered K(+) channel-dependent module for electrical excitability of the vacuolar membrane.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Dindas, J. and Dreyer, I. and Huang, S. and Hedrich, R. and Roelfsema, M. R. G.}, biburl = {https://www.bibsonomy.org/bibtex/24487e88c18ac5933ec596feee5a0fa0c/jvsi_all}, doi = {10.1111/nph.17272}, interhash = {8c04dcb2ee9dd5ad1e6dc9947298ce66}, intrahash = {4487e88c18ac5933ec596feee5a0fa0c}, issn = {1469-8137 (Electronic) 0028-646X (Linking)}, journal = {New Phytol}, keywords = {*Arabidopsis/metabolism myOwn}, note = {Dindas, Julian Dreyer, Ingo Huang, Shouguang Hedrich, Rainer Roelfsema, M Rob G eng Research Support, Non-U.S. Gov't England 2021/02/13 New Phytol. 2021 May;230(4):1449-1460. doi: 10.1111/nph.17272. Epub 2021 Mar 30.}, number = 4, pages = {1449-1460}, timestamp = {2024-02-15T15:08:22.000+0100}, title = {A voltage-dependent Ca(2+) homeostat operates in the plant vacuolar membrane}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/33577135}, volume = 230, year = 2021 }