%0 Journal Article %1 Yakovlev1994 %A Yakovlev, A. G. %A Faden, A. I. %D 1994 %J Mol Chem Neuropathol %K , Sprague-Dawley; Animals; Base Sequence; Dynorphins, biosynthesis/genetics; Gene Expression, physiology; Genes, fos, drug effects; Histones, Male; Molecular Sequence Data; Polymerase Chain Reaction; Protein Precursors, RNA, Messenger, biosynthesis; Rats; Spinal Cord Injuries, metabolism; Transcription, Genetic; Tumor Necrosis Factor-alpha, biosynthesis/genetics %N 2-3 %P 179--190 %T Sequential expression of c-fos protooncogene, TNF-alpha, and dynorphin genes in spinal cord following experimental traumatic injury. %V 23 %X Reverse transcription-polymerase chain reaction (RT-PCR) was used to estimate dynamic changes in levels of c-fos protooncogene, tumor necrosis factor alpha (TNF-alpha), and preprodynorphin messenger ribonucleic acid (mRNA) isolated from individual segments (T1 to T12) of rat spinal cord following graded impact trauma (50 or 100 g/cm) to the T9 segment of pentobarbital-anesthetized rats. Trauma caused elevation of c-fos mRNA at the trauma site by 30 min after injury that was related to injury severity. At this time, increased levels of TNF-alpha (but not of preprodynorphin) mRNA were also found. By 24 h, c-fos and TNF-alpha mRNA had returned to normal levels at trauma site, but were now increased at more distal segments (T5 and T12). At 4 h after trauma, induction of preprodynorphin mRNA was detected at the trauma site; levels continued to be elevated at 24 h when they were also detected at T5 and T12. Increases for each mRNA were greater for severe as compared to moderate trauma. The injury dose- and time-dependent changes in c-fos, TNF-alpha, and preprodynorphin gene expression suggest that their respective proteins are synthesized in response to trauma, and may play a part in the secondary injury response. Later accumulation of message distant from the trauma site may reflect a progression of delayed damage along the spinal cord.

@article{Yakovlev1994, abstract = {Reverse transcription-polymerase chain reaction (RT-PCR) was used to estimate dynamic changes in levels of c-fos protooncogene, tumor necrosis factor alpha (TNF-alpha), and preprodynorphin messenger ribonucleic acid (mRNA) isolated from individual segments (T1 to T12) of rat spinal cord following graded impact trauma (50 or 100 g/cm) to the T9 segment of pentobarbital-anesthetized rats. Trauma caused elevation of c-fos mRNA at the trauma site by 30 min after injury that was related to injury severity. At this time, increased levels of TNF-alpha (but not of preprodynorphin) mRNA were also found. By 24 h, c-fos and TNF-alpha mRNA had returned to normal levels at trauma site, but were now increased at more distal segments (T5 and T12). At 4 h after trauma, induction of preprodynorphin mRNA was detected at the trauma site; levels continued to be elevated at 24 h when they were also detected at T5 and T12. Increases for each mRNA were greater for severe as compared to moderate trauma. The injury dose- and time-dependent changes in c-fos, TNF-alpha, and preprodynorphin gene expression suggest that their respective proteins are synthesized in response to trauma, and may play a part in the secondary injury response. Later accumulation of message distant from the trauma site may reflect a progression of delayed damage along the spinal cord.}, added-at = {2014-07-19T17:15:28.000+0200}, author = {Yakovlev, A. G. and Faden, A. I.}, biburl = {https://www.bibsonomy.org/bibtex/2751563b79f4a0e41fc7acb584a3f23df/ar0berts}, groups = {public}, institution = {Department of Neurology, Georgetown University Medical Center, Washington, DC 20007.}, interhash = {8cc32f9690438621820f96d9de63e3a2}, intrahash = {751563b79f4a0e41fc7acb584a3f23df}, journal = {Mol Chem Neuropathol}, keywords = {, Sprague-Dawley; Animals; Base Sequence; Dynorphins, biosynthesis/genetics; Gene Expression, physiology; Genes, fos, drug effects; Histones, Male; Molecular Sequence Data; Polymerase Chain Reaction; Protein Precursors, RNA, Messenger, biosynthesis; Rats; Spinal Cord Injuries, metabolism; Transcription, Genetic; Tumor Necrosis Factor-alpha, biosynthesis/genetics}, language = {eng}, medline-pst = {ppublish}, number = {2-3}, pages = {179--190}, pmid = {7702707}, timestamp = {2014-07-19T17:15:28.000+0200}, title = {Sequential expression of c-fos protooncogene, TNF-alpha, and dynorphin genes in spinal cord following experimental traumatic injury.}, username = {ar0berts}, volume = 23, year = 1994 }