%0 Journal Article %1 Bates2007 %A Bates, Mark %A Huang, Bo %A Dempsey, Graham T. %A Zhuang, Xiaowei %D 2007 %I American Association for the Advancement of Science %J Science %K fluorophores microscopy storm superresolution %N 5845 %P 1749--1753 %R 10.1126/science.1146598 %T Multicolor super-resolution imaging with photo-switchable fluorescent probes %U http://science.sciencemag.org/ %V 317 %X Recent advances in far-field optical nanoscopy have enabled fluorescence imaging with a spatial resolution of 20 to 50 nanometers. Multicolor super-resolution imaging, however, remains a challenging task. Here, we introduce a family of photo-switchable fluorescent probes and demonstrate multicolor stochastic optical reconstruction microscopy (STORM). Each probe consists of a photo-switchable "reporter" fluorophore that can be cycled between fluorescent and dark states, and an äctivator" that facilitates photo-activation of the reporter. Combinatorial pairing of reporters and activators allows the creation of probes with many distinct colors. Iterative, color-specific activation of sparse subsets of these probes allows their localization with nanometer accuracy, enabling the construction of a super-resolution STORM image. Using this approach, we demonstrate multicolor imaging of DNA model samples and mammalian cells with 20- to 30-nanometer resolution. This technique will facilitate direct visualization of molecular interactions at the nanometer scale.

@article{Bates2007, abstract = {Recent advances in far-field optical nanoscopy have enabled fluorescence imaging with a spatial resolution of 20 to 50 nanometers. Multicolor super-resolution imaging, however, remains a challenging task. Here, we introduce a family of photo-switchable fluorescent probes and demonstrate multicolor stochastic optical reconstruction microscopy (STORM). Each probe consists of a photo-switchable "reporter" fluorophore that can be cycled between fluorescent and dark states, and an "activator" that facilitates photo-activation of the reporter. Combinatorial pairing of reporters and activators allows the creation of probes with many distinct colors. Iterative, color-specific activation of sparse subsets of these probes allows their localization with nanometer accuracy, enabling the construction of a super-resolution STORM image. Using this approach, we demonstrate multicolor imaging of DNA model samples and mammalian cells with 20- to 30-nanometer resolution. This technique will facilitate direct visualization of molecular interactions at the nanometer scale.}, added-at = {2020-03-23T21:12:34.000+0100}, author = {Bates, Mark and Huang, Bo and Dempsey, Graham T. and Zhuang, Xiaowei}, biburl = {https://www.bibsonomy.org/bibtex/2fae5cf41dd5f23aa7d5051ed1cd57601/kfriedl}, doi = {10.1126/science.1146598}, file = {:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Bates et al. - Unknown - Multicolor Super-Resolution Imaging with Photo-Switchable Fluorescent Probes.pdf:pdf}, interhash = {909528252b5aaabcafd0e9df0e40bc5e}, intrahash = {fae5cf41dd5f23aa7d5051ed1cd57601}, issn = {00368075}, journal = {Science}, keywords = {fluorophores microscopy storm superresolution}, month = sep, number = 5845, pages = {1749--1753}, publisher = {American Association for the Advancement of Science}, timestamp = {2020-03-23T21:36:23.000+0100}, title = {{Multicolor super-resolution imaging with photo-switchable fluorescent probes}}, url = {http://science.sciencemag.org/}, volume = 317, year = 2007 }