%0 Journal Article %1 golumbek1993controlled %A Golumbek, Paul T %A Azhari, Rosa %A Jaffee, Elizabeth M %A Levitsky, Hyam I %A Lazenby, Audrey %A Leong, Kam %A Pardoll, Drew M %D 1993 %J Cancer Research %K cancer history immuneSystem methods vaccine %P 5841-5844 %T Controlled Release, Biodegradable Cytokine Depots: A New Approach in Cancer Vaccine Design %U https://pdfs.semanticscholar.org/39d7/7fb7ea59c6900c8e05d504c7258c31d55ec7.pdf %V 53 %X Experimental studies using murine tumor models have demonstrated that potent systemic immunity can be generated using tumor vaccines engineered by gene transfer to secrete certain cytokines. The underlying physiological principle behind these strategies involves the sustained re lease of high doses of cytokine at the site of the tumor. In some cases, this paracrine approach appears to enhance tumor antigen presentation and avoids systemic cytokine toxicity. The widespread clinical use of autologous cytokine gene transduced tumor vaccines may be limited by the technical difficulty and labor intensity of individualized gene transfer. We have therefore explored an alternate approach to generating sustained release of cytokines local to the tumor cells. High doses of granulocytemacrophage colony-stimulating factor encapsulated in cell-sized gelatinchondroitin sulfate microspheres were mixed with irradiated tumor cells prior to s.c. injection. This vaccination scheme resulted in systemic antitumor immune responses comparable to granulocyte-macrophage colonystimulating factor gene transduced tumor vaccines.

@article{golumbek1993controlled, abstract = {Experimental studies using murine tumor models have demonstrated that potent systemic immunity can be generated using tumor vaccines engineered by gene transfer to secrete certain cytokines. The underlying physiological principle behind these strategies involves the sustained re lease of high doses of cytokine at the site of the tumor. In some cases, this paracrine approach appears to enhance tumor antigen presentation and avoids systemic cytokine toxicity. The widespread clinical use of autologous cytokine gene transduced tumor vaccines may be limited by the technical difficulty and labor intensity of individualized gene transfer. We have therefore explored an alternate approach to generating sustained release of cytokines local to the tumor cells. High doses of granulocytemacrophage colony-stimulating factor encapsulated in cell-sized gelatinchondroitin sulfate microspheres were mixed with irradiated tumor cells prior to s.c. injection. This vaccination scheme resulted in systemic antitumor immune responses comparable to granulocyte-macrophage colonystimulating factor gene transduced tumor vaccines.}, added-at = {2017-12-02T23:17:54.000+0100}, author = {Golumbek, Paul T and Azhari, Rosa and Jaffee, Elizabeth M and Levitsky, Hyam I and Lazenby, Audrey and Leong, Kam and Pardoll, Drew M}, biburl = {https://www.bibsonomy.org/bibtex/2ba39ca4795c89e1ccd87245dc56d2018/artheibault}, description = {This article describes another early attempt to induce the immune system to fight cancer. In this case, the researchers use cytokines to control tumor growth. Because systemic cytokine induction is highly toxic, the researchers released microspheres containing cytokine capsules into the bloodstream of the mice. The cytokines appeared to have a tumor-suppressing effect.}, interhash = {91ec722ba180f45cd9b46f3acaf4c3f3}, intrahash = {ba39ca4795c89e1ccd87245dc56d2018}, journal = {Cancer Research}, keywords = {cancer history immuneSystem methods vaccine}, month = dec, pages = {5841-5844}, timestamp = {2017-12-02T23:17:54.000+0100}, title = {Controlled Release, Biodegradable Cytokine Depots: A New Approach in Cancer Vaccine Design}, url = {https://pdfs.semanticscholar.org/39d7/7fb7ea59c6900c8e05d504c7258c31d55ec7.pdf}, volume = 53, year = 1993 }