%0 Journal Article %1 jaslan2019voltagedependent %A Jaslan, D. %A Dreyer, I. %A Lu, J. %A O'Malley, R. %A Dindas, J. %A Marten, I. %A Hedrich, R. %D 2019 %J Nat Commun %K Arabidopsis/*physiology myOwn %N 1 %P 2659 %R 10.1038/s41467-019-10599-x %T Voltage-dependent gating of SV channel TPC1 confers vacuole excitability %U https://www.ncbi.nlm.nih.gov/pubmed/31201323 %V 10 %X In contrast to the plasma membrane, the vacuole membrane has not yet been associated with electrical excitation of plants. Here, we show that mesophyll vacuoles from Arabidopsis sense and control the membrane potential essentially via the K(+)-permeable TPC1 and TPK channels. Electrical stimuli elicit transient depolarization of the vacuole membrane that can last for seconds. Electrical excitability is suppressed by increased vacuolar Ca(2+) levels. In comparison to wild type, vacuoles from the fou2 mutant, harboring TPC1 channels insensitive to luminal Ca(2+), can be excited fully by even weak electrical stimuli. The TPC1-loss-of-function mutant tpc1-2 does not respond to electrical stimulation at all, and the loss of TPK1/TPK3-mediated K(+) transport affects the duration of TPC1-dependent membrane depolarization. In combination with mathematical modeling, these results show that the vacuolar K(+)-conducting TPC1 and TPK1/TPK3 channels act in concert to provide for Ca(2+)- and voltage-induced electrical excitability to the central organelle of plant cells.

@article{jaslan2019voltagedependent, abstract = {In contrast to the plasma membrane, the vacuole membrane has not yet been associated with electrical excitation of plants. Here, we show that mesophyll vacuoles from Arabidopsis sense and control the membrane potential essentially via the K(+)-permeable TPC1 and TPK channels. Electrical stimuli elicit transient depolarization of the vacuole membrane that can last for seconds. Electrical excitability is suppressed by increased vacuolar Ca(2+) levels. In comparison to wild type, vacuoles from the fou2 mutant, harboring TPC1 channels insensitive to luminal Ca(2+), can be excited fully by even weak electrical stimuli. The TPC1-loss-of-function mutant tpc1-2 does not respond to electrical stimulation at all, and the loss of TPK1/TPK3-mediated K(+) transport affects the duration of TPC1-dependent membrane depolarization. In combination with mathematical modeling, these results show that the vacuolar K(+)-conducting TPC1 and TPK1/TPK3 channels act in concert to provide for Ca(2+)- and voltage-induced electrical excitability to the central organelle of plant cells.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Jaslan, D. and Dreyer, I. and Lu, J. and O'Malley, R. and Dindas, J. and Marten, I. and Hedrich, R.}, biburl = {https://www.bibsonomy.org/bibtex/25ad021520835b7f56655793708336897/jvsi_all}, doi = {10.1038/s41467-019-10599-x}, interhash = {95356000952179dba993dc6bf10b3256}, intrahash = {5ad021520835b7f56655793708336897}, issn = {2041-1723 (Electronic) 2041-1723 (Linking)}, journal = {Nat Commun}, keywords = {Arabidopsis/*physiology myOwn}, note = {Jaslan, Dawid Dreyer, Ingo Lu, Jinping O'Malley, Ronan Dindas, Julian Marten, Irene Hedrich, Rainer eng FOR1061/He1640/Deutsche Forschungsgemeinschaft (German Research Foundation)/International Research Support, Non-U.S. Gov't England 2019/06/16 Nat Commun. 2019 Jun 14;10(1):2659. doi: 10.1038/s41467-019-10599-x.}, number = 1, pages = 2659, timestamp = {2024-02-15T15:08:22.000+0100}, title = {Voltage-dependent gating of SV channel TPC1 confers vacuole excitability}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/31201323}, volume = 10, year = 2019 }