%0 Journal Article %1 MARTINSEN1991 %A MARTINSEN, A. %A SKJAKBRAEK, G. %A SMIDSROD, O. %A ZANETTI, F. %A PAOLETTI, S. %C THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB %D 1991 %I ELSEVIER SCI LTD %J Carbohydr. Polym. %K ;; [ISI:] alginate light-scattering; sodium %N 2 %P 171 -- 193 %T COMPARISON OF DIFFERENT METHODS FOR DETERMINATION OF MOLECULAR-WEIGHT AND MOLECULAR-WEIGHT DISTRIBUTION OF ALGINATES %V 15 %X The scope of the present work was to assess and compare different methods to determine molecular weight (MW) averages and molecular weight distributions (MWD) of two samples of sodium alginate of different composition. Weight average (MBAR(w)) and number average (MBAR(n)) molecular weights were determined by totally integrated laser light scattering (LLS), both at wide angles (WA) and at a low angle (LA), and membrane osmometry, respectively. The two alginates were fractionated by preparative and analytical size exclusion chromatography. Absolute molecular weight distribution curves were obtained from SEC, using an on-line LA-LLS detector coupled with an RI concentration detector. Relative MWD curves were determined from 'universal' calibration of the SEC data. MBAR(w), MBAR(v), and MBAR(n) were calculated from the 'universal' calibration curve and etaj values of the fractions. Good correlation between molecular weight averages obtained from the different methods was observed. Mark-Houwink-Sakurada parameters, a and K, for the two different alginate samples have been determined.

@article{MARTINSEN1991, __markedentry = {[phpts:6]}, abstract = {The scope of the present work was to assess and compare different methods to determine molecular weight (MW) averages and molecular weight distributions (MWD) of two samples of sodium alginate of different composition. Weight average (MBAR(w)) and number average (MBAR(n)) molecular weights were determined by totally integrated laser light scattering (LLS), both at wide angles (WA) and at a low angle (LA), and membrane osmometry, respectively. The two alginates were fractionated by preparative and analytical size exclusion chromatography. Absolute molecular weight distribution curves were obtained from SEC, using an on-line LA-LLS detector coupled with an RI concentration detector. Relative MWD curves were determined from 'universal' calibration of the SEC data. MBAR(w), MBAR(v), and MBAR(n) were calculated from the 'universal' calibration curve and [eta]j values of the fractions. Good correlation between molecular weight averages obtained from the different methods was observed. Mark-Houwink-Sakurada parameters, a and K, for the two different alginate samples have been determined.}, added-at = {2011-11-04T13:47:04.000+0100}, address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, OXON, ENGLAND OX5 1GB}, author = {MARTINSEN, A. and SKJAKBRAEK, G. and SMIDSROD, O. and ZANETTI, F. and PAOLETTI, S.}, authoraddress = {UNIV TRIESTE,DEPT BIOCHEM BIOPHYS & MACROMOLEC CHEM,P EUROPA 1,I-34127 TRIESTE,ITALY. ; NORWEGIAN INST TECHNOL,DIV BIOTECHNOL,NORWEGIAN BIOPOLYMER LAB,N-7034 TRONDHEIM,NORWAY. ; POLY BIOS RES CTR,TECHNOL BIOPOLYMERS LAB,I-34012 TRIESTE,ITALY.}, biburl = {https://www.bibsonomy.org/bibtex/23545836317783709672828ec463a3ff8/pawelsikorski}, citedref = {BALL A, 1988, INT J BIOL MACROMOL, V10, P259 ; DINGSOYR E, 1977, BRIT POLYM J, V9, P56 ; DUBOIS M, 1956, ANAL CHEM, V28, P350 ; GRASDALEN H, 1979, CARBOHYD RES, V68, P23 ; GRASDALEN H, 1983, CARBOHYD RES, V118, P255 ; HARDING S, IN PRESS ADV CARBOHY, V1 ; HAUG A, 1959, ACTA CHEM SCAND, V13, P1250 ; HAUG A, 1966, ACTA CHEM SCAND, V20, P183 ; LIM F, 1980, SCIENCE, V210, P908 ; MACKIE W, 1980, BIOPOLYMERS, V19, P1839 ; MAHN B, 1984, LSN113 LDC MILT ROY ; MARTINSEN A, 1989, BIOTECHNOL BIOENG, V33, P79 ; MCCONNELL ML, 1978, AM LAB, V10, P63 ; SKJAKBRAEK G, 1989, CARBOHYD POLYM, V10, P31 ; SKJAKBRAEK G, 1990, SEAWEED RESOURCES EU ; SMIDSROD O, 1968, ACTA CHEM SCAND, V22, P797 ; STRAND KA, 1982, MACROMOLECULES, V15, P570 ; WEDLOCK DJ, 1986, INT J BIOL MACROMOL, V8, P57}, interhash = {960d82f753b8eb8f2f12ee9ab6a79a73}, intrahash = {3545836317783709672828ec463a3ff8}, isifile-dt = {Article}, isifile-ga = {FG161}, isifile-j9 = {CARBOHYD POLYM}, isifile-nr = {18}, isifile-pi = {OXFORD}, isifile-sc = {Chemistry, Applied; Chemistry, Organic; Polymer Science}, isifile-tc = {66}, issn = {0144-8617}, journal = {Carbohydr. Polym.}, keywords = {;; [ISI:] alginate light-scattering; sodium}, language = {English}, number = 2, owner = {phpts}, pages = {171 -- 193}, publisher = {ELSEVIER SCI LTD}, size = {23 p.}, sourceid = {ISI:A1991FG16100004}, timestamp = {2011-11-04T13:47:17.000+0100}, title = {COMPARISON OF DIFFERENT METHODS FOR DETERMINATION OF MOLECULAR-WEIGHT AND MOLECULAR-WEIGHT DISTRIBUTION OF ALGINATES}, volume = 15, year = 1991 }