%0 Generic %1 assembly %A Tao, Y %A Olson, N H %A Xu, W %A Anderson, D L %A Rossmann, M G %A Baker, T S %D 1998 %I Cell Press %J Cell %K phage %N 3 %P 431--437 %T Assembly of a Tailed Bacterial Virus and Its Genome Release Studied in Three Dimensions %U http://linkinghub.elsevier.com/retrieve/pii/S0092867400817730 %V 95 %X We present the first three-dimensional reconstruction of a prolate, tailed phage, and its empty prohead precursor by cryo-electron microscopy. The headtail connector, the central component of the DNA packaging machine, is visualized for the first time in situ within the Bacillus subtilis dsDNA phage �29. The connector, with 12- or 13-fold symmetry, appears to fit loosely into a pentameric vertex of the head, a symmetry mismatch that may be required to rotate the connector to package DNA. The prolate head of �29 has 10 hexameric units in its cylindrical equatorial region, and 11 pentameric and 20 hexameric units comprise icosahedral end-caps with T=3 quasi-symmetry. Reconstruction of an emptied phage particle shows that the connector and neck/tail assembly undergo significant conformational changes upon ejection of DNA.

@misc{assembly, abstract = {We present the first three-dimensional reconstruction of a prolate, tailed phage, and its empty prohead precursor by cryo-electron microscopy. The headtail connector, the central component of the DNA packaging machine, is visualized for the first time in situ within the Bacillus subtilis dsDNA phage �29. The connector, with 12- or 13-fold symmetry, appears to fit loosely into a pentameric vertex of the head, a symmetry mismatch that may be required to rotate the connector to package DNA. The prolate head of �29 has 10 hexameric units in its cylindrical equatorial region, and 11 pentameric and 20 hexameric units comprise icosahedral end-caps with T=3 quasi-symmetry. Reconstruction of an emptied phage particle shows that the connector and neck/tail assembly undergo significant conformational changes upon ejection of DNA.}, added-at = {2014-02-22T13:26:52.000+0100}, author = {Tao, Y and Olson, N H and Xu, W and Anderson, D L and Rossmann, M G and Baker, T S}, biburl = {https://www.bibsonomy.org/bibtex/20da4fc09d6dc33244b68d74292aa765a/ross_mck}, description = {Cell - Assembly of a Tailed Bacterial Virus and Its Genome Release Studied in Three Dimensions}, interhash = {9bc4256da104d983d257c52ff2d19066}, intrahash = {0da4fc09d6dc33244b68d74292aa765a}, issn = {00928674}, journal = {Cell}, keywords = {phage}, month = oct, number = 3, pages = {431--437}, publisher = {Cell Press}, refid = {S0092-8674(00)81773-0 DOI -}, timestamp = {2014-02-22T13:45:09.000+0100}, title = {Assembly of a Tailed Bacterial Virus and Its Genome Release Studied in Three Dimensions}, url = {http://linkinghub.elsevier.com/retrieve/pii/S0092867400817730}, volume = 95, year = 1998 }