%0 Journal Article %1 Hein2009 %A Hein, P. %A Bunemann, M. %D 2009 %J Naunyn Schmiedebergs Arch Pharmacol %K *Models, *Signal Animals Binding Biological G-Protein-Coupled/*metabolism/physiology GTP-Binding Humans Protein Proteins/*metabolism/physiology Transduction Receptor %N 5 %P 435-43 %T Coupling mode of receptors and G proteins %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19048232 %V 379 %X Signaling via G-protein-coupled receptors (GPCRs) is crucial to many physiological and pathophysiological processes in multicellular organisms, and GPCRs themselves are targets for important drugs. Classical cell supplementation experiments suggest a collision coupling model, in which receptors and G proteins diffuse randomly within the cell membrane and interact only if receptors are activated. This model is also backed by kinetic and live cell imaging data. According to the challenging theory, receptors and G proteins are precoupled--meaning they are forming stable complexes in the absence of agonist, which prevail during signaling. This model has been favored on the basis of copurification and coimmunoprecipitation of inactive receptors with G proteins and more recently by some approaches measuring energy transfer between labeled receptors and G proteins. This article reviews key findings regarding the receptor/G protein coupling mode, including most recent findings obtained by optical techniques.

@article{Hein2009, abstract = {Signaling via G-protein-coupled receptors (GPCRs) is crucial to many physiological and pathophysiological processes in multicellular organisms, and GPCRs themselves are targets for important drugs. Classical cell supplementation experiments suggest a collision coupling model, in which receptors and G proteins diffuse randomly within the cell membrane and interact only if receptors are activated. This model is also backed by kinetic and live cell imaging data. According to the challenging theory, receptors and G proteins are precoupled--meaning they are forming stable complexes in the absence of agonist, which prevail during signaling. This model has been favored on the basis of copurification and coimmunoprecipitation of inactive receptors with G proteins and more recently by some approaches measuring energy transfer between labeled receptors and G proteins. This article reviews key findings regarding the receptor/G protein coupling mode, including most recent findings obtained by optical techniques.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Hein, P. and Bunemann, M.}, biburl = {https://www.bibsonomy.org/bibtex/2dcc4cda9427284746c63f2654ca6b8d6/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {9c6712164bee57b77c71db02daa36546}, intrahash = {dcc4cda9427284746c63f2654ca6b8d6}, issn = {1432-1912 (Electronic) 1432-1912 (Linking)}, journal = {Naunyn Schmiedebergs Arch Pharmacol}, keywords = {*Models, *Signal Animals Binding Biological G-Protein-Coupled/*metabolism/physiology GTP-Binding Humans Protein Proteins/*metabolism/physiology Transduction Receptor}, month = May, note = {Hein, Peter Bunemann, Moritz Research Support, Non-U.S. Gov't Review Germany Naunyn-Schmiedeberg's archives of pharmacology Naunyn Schmiedebergs Arch Pharmacol. 2009 May;379(5):435-43. Epub 2008 Dec 2.}, number = 5, pages = {435-43}, shorttitle = {Coupling mode of receptors and G proteins}, timestamp = {2010-12-14T18:20:03.000+0100}, title = {Coupling mode of receptors and G proteins}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19048232}, volume = 379, year = 2009 }