%0 Journal Article %1 Gohla1999 %A Gohla, A. %A Offermanns, S. %A Wilkie, T. M. %A Schultz, G. %D 1999 %J J Biol Chem %K *GTP-Binding AMP/metabolism Actins/*metabolism Animals Calcium/metabolism Cell Cyclic Cytoskeleton/*metabolism Epidermal Factor/metabolism Fibroblasts GTP-Binding Gi-Go Gi2 Gohla Growth Knockout Ligands Lysophospholipids/physiology Mice Microinjections Protein Proteins/genetics/*metabolism Proto-Oncogene Signal Subunit, Subunits, Surface/*metabolism Thrombin/pharmacology Transduction Tyrphostins/pharmacology alpha Receptor %N 25 %P 17901-7 %T Differential involvement of Galpha12 and Galpha13 in receptor-mediated stress fiber formation %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10364236 %V 274 %X The ubiquitously expressed heterotrimeric guanine nucleotide-binding proteins (G-proteins) G12 and G13 have been shown to activate the small GTPase Rho. Rho stimulation leads to a rapid remodeling of the actin cytoskeleton and subsequent stress fiber formation. We investigated the involvement of G12 or G13 in stress fiber formation induced through a variety of Gq/G11-coupled receptors. Using fibroblast cell lines derived from wild-type and Galphaq/Galpha11-deficient mice, we show that agonist-dependent activation of the endogenous receptors for thrombin or lysophosphatidic acid and of the heterologously expressed bradykinin B2, vasopressin V1A, endothelin ETA, and serotonin 5-HT2C receptors induced stress fiber formation in either the presence or absence of Galphaq/Galpha11. Stress fiber assembly induced through the muscarinic M1 and the metabotropic glutamate subtype 1alpha receptors was dependent on Gq/G11 proteins. The activation of the Gq/G11-coupled endothelin ETB and angiotensin AT1A receptors failed to induce stress fiber formation. Lysophosphatidic acid, B2, and 5-HT2C receptor-mediated stress fiber formation was dependent on Galpha13 and involved epidermal growth factor (EGF) receptors, whereas thrombin, ETA, and V1A receptors induced stress fiber accumulation via Galpha12 in an EGF receptor-independent manner. Our data demonstrate that many Gq/G11-coupled receptors induce stress fiber assembly in the absence of Galphaq and Galpha11 and that this involves either a Galpha12 or a Galpha13/EGF receptor-mediated pathway.

@article{Gohla1999, abstract = {The ubiquitously expressed heterotrimeric guanine nucleotide-binding proteins (G-proteins) G12 and G13 have been shown to activate the small GTPase Rho. Rho stimulation leads to a rapid remodeling of the actin cytoskeleton and subsequent stress fiber formation. We investigated the involvement of G12 or G13 in stress fiber formation induced through a variety of Gq/G11-coupled receptors. Using fibroblast cell lines derived from wild-type and Galphaq/Galpha11-deficient mice, we show that agonist-dependent activation of the endogenous receptors for thrombin or lysophosphatidic acid and of the heterologously expressed bradykinin B2, vasopressin V1A, endothelin ETA, and serotonin 5-HT2C receptors induced stress fiber formation in either the presence or absence of Galphaq/Galpha11. Stress fiber assembly induced through the muscarinic M1 and the metabotropic glutamate subtype 1alpha receptors was dependent on Gq/G11 proteins. The activation of the Gq/G11-coupled endothelin ETB and angiotensin AT1A receptors failed to induce stress fiber formation. Lysophosphatidic acid, B2, and 5-HT2C receptor-mediated stress fiber formation was dependent on Galpha13 and involved epidermal growth factor (EGF) receptors, whereas thrombin, ETA, and V1A receptors induced stress fiber accumulation via Galpha12 in an EGF receptor-independent manner. Our data demonstrate that many Gq/G11-coupled receptors induce stress fiber assembly in the absence of Galphaq and Galpha11 and that this involves either a Galpha12 or a Galpha13/EGF receptor-mediated pathway.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Gohla, A. and Offermanns, S. and Wilkie, T. M. and Schultz, G.}, biburl = {https://www.bibsonomy.org/bibtex/21c86aa596fe2e1a475121702b52c9ef7/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {9de8e44d27501b1524d62b748b2c0e17}, intrahash = {1c86aa596fe2e1a475121702b52c9ef7}, issn = {0021-9258 (Print) 0021-9258 (Linking)}, journal = {J Biol Chem}, keywords = {*GTP-Binding AMP/metabolism Actins/*metabolism Animals Calcium/metabolism Cell Cyclic Cytoskeleton/*metabolism Epidermal Factor/metabolism Fibroblasts GTP-Binding Gi-Go Gi2 Gohla Growth Knockout Ligands Lysophospholipids/physiology Mice Microinjections Protein Proteins/genetics/*metabolism Proto-Oncogene Signal Subunit, Subunits, Surface/*metabolism Thrombin/pharmacology Transduction Tyrphostins/pharmacology alpha Receptor}, month = {Jun 18}, note = {Gohla, A Offermanns, S Wilkie, T M Schultz, G Research Support, Non-U.S. Gov't United states The Journal of biological chemistry J Biol Chem. 1999 Jun 18;274(25):17901-7.}, number = 25, pages = {17901-7}, shorttitle = {Differential involvement of Galpha12 and Galpha13 in receptor-mediated stress fiber formation}, timestamp = {2010-12-14T18:21:25.000+0100}, title = {Differential involvement of Galpha12 and Galpha13 in receptor-mediated stress fiber formation}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10364236}, volume = 274, year = 1999 }