%0 Journal Article %1 citeulike:488366 %A Chambers, Jill A. %A Walton, Nicholas J. %D 1988 %J Journal of Electroanalytical Chemistry %K displacement amperometry labeled_mediator immunoassay homogeneous immunosensor ag_detection electrochemistry %N 2 %P 417--425 %R 10.1016/0022-0728(88)85181-7 %T The development of redox-modified electrodes as charge-accumulating devices for use in higher sensitivity detection systems %U http://www.sciencedirect.com/science/article/B6TGB-44XVYNV-12/2/7b60c76c37321dc6ddd0d739d1b6fe78 %V 250 %X Electrochemical methods can be applied in a number of ways as detection systems for competitive immunoassays, especially through the use of redox-labelled antigens. To overcome the problem of detection of such conjugates at low concentration, a system is described in which a poly(vinylferrocene)-modified glassy carbon electrode is used to accumulate charge from solution glucose/glucose oxidase via the intermediacy of a redox-labelled antigen, cycled repeatedly between the enzyme and the electrode. The charge accumulated by the electrode is measured electrochemically by returning the electrode to its original potential and integrating the resulting current-time transient. The charge is proportional to the concentration of redox-labelled antigen in solution and to the charge-accumulation time. In this way 1,1'-dimethylferrocene-ethan-1-ol-2-amine was reproducibly detected to 0.9 nM, with a 10 min incubation period. The 8-ferrocenyl conjugate of theophylline was characterised as a model system for possible application in a higher sensitivity electrochemical immunoassay.

@article{citeulike:488366, abstract = {Electrochemical methods can be applied in a number of ways as detection systems for competitive immunoassays, especially through the use of redox-labelled antigens. To overcome the problem of detection of such conjugates at low concentration, a system is described in which a poly(vinylferrocene)-modified glassy carbon electrode is used to accumulate charge from solution glucose/glucose oxidase via the intermediacy of a redox-labelled antigen, cycled repeatedly between the enzyme and the electrode. The charge accumulated by the electrode is measured electrochemically by returning the electrode to its original potential and integrating the resulting current-time transient. The charge is proportional to the concentration of redox-labelled antigen in solution and to the charge-accumulation time. In this way 1,1'-dimethylferrocene-ethan-1-ol-2-amine was reproducibly detected to 0.9 nM, with a 10 min incubation period. The 8-ferrocenyl conjugate of theophylline was characterised as a model system for possible application in a higher sensitivity electrochemical immunoassay.}, added-at = {2006-07-07T01:10:50.000+0200}, author = {Chambers, Jill A. and Walton, Nicholas J.}, biburl = {https://www.bibsonomy.org/bibtex/2a7095ed0d11a2eb7de0d671cf908559d/biblio24}, citeulike-article-id = {488366}, doi = {10.1016/0022-0728(88)85181-7}, interhash = {9f83c4c00ce3df7702d5db4f6f94da82}, intrahash = {a7095ed0d11a2eb7de0d671cf908559d}, journal = {Journal of Electroanalytical Chemistry}, keywords = {displacement amperometry labeled_mediator immunoassay homogeneous immunosensor ag_detection electrochemistry}, month = {August}, number = 2, pages = {417--425}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {The development of redox-modified electrodes as charge-accumulating devices for use in higher sensitivity detection systems}, url = {http://www.sciencedirect.com/science/article/B6TGB-44XVYNV-12/2/7b60c76c37321dc6ddd0d739d1b6fe78}, volume = 250, year = 1988 }