%0 Journal Article %1 gelmedin_echinococcus_2005 %A Gelmedin, Verena %A Zavala-Góngora, Ricardo %A Fernández, Cecilia %A Brehm, Klaus %D 2005 %J Experimental Parasitology %K Acid Acid, Alignment, Amino Animals, Base Chain Cloning, Complementary, Data, Echinococcus Helminth, Homology, Molecular Molecular, Nuclear Polymerase Proteins, Reaction, Reverse Sequence Sequence, Transcriptase ag_brehm multilocularis, {DNA,} {Trans-Activators} %N 2 %P 115--120 %R 10.1016/j.exppara.2005.04.004 %T Echinococcus multilocularis: cloning and characterization of a member of the SNW/SKIP family of transcriptional coregulators %U http://www.ncbi.nlm.nih.gov/pubmed/15936017 %V 111 %X We have isolated a cDNA from the fox-tapeworm Echinococcus multilocularis that encodes EmSkip, a novel member of the SNW/SKIP family of transcriptional coregulators. EmSkip displays significant amino acid sequence homologies to already known members of the protein family and contains all the characteristic amino acid residues at their corresponding positions. RT-PCR experiments showed that the EmSkip encoding gene, emskip, is expressed in the Echinococcus larval stages metacestode and protoscolex during an infection of the intermediate host. By yeast two-hybrid analyses, EmSkip was found to be capable of forming homodimers in vivo. Furthermore, EmSkip was found to interact with EmSmadA and EmSmadB, two previously identified TGF-beta/BMP signal transducers of E. multilocularis, indicating a role of this protein in TGF-beta signaling processes in the parasite. In view of the role played by SNW/SKIP proteins in splicing mechanisms and intracellular signaling, the data presented herein should facilitate the identification of Echinococcus factors involved in such processes.

@article{gelmedin_echinococcus_2005, abstract = {We have isolated a {cDNA} from the fox-tapeworm Echinococcus multilocularis that encodes {EmSkip,} a novel member of the {SNW/SKIP} family of transcriptional coregulators. {EmSkip} displays significant amino acid sequence homologies to already known members of the protein family and contains all the characteristic amino acid residues at their corresponding positions. {RT-PCR} experiments showed that the {EmSkip} encoding gene, emskip, is expressed in the Echinococcus larval stages metacestode and protoscolex during an infection of the intermediate host. By yeast two-hybrid analyses, {EmSkip} was found to be capable of forming homodimers in vivo. Furthermore, {EmSkip} was found to interact with {EmSmadA} and {EmSmadB,} two previously identified {TGF-beta/BMP} signal transducers of E. multilocularis, indicating a role of this protein in {TGF-beta} signaling processes in the parasite. In view of the role played by {SNW/SKIP} proteins in splicing mechanisms and intracellular signaling, the data presented herein should facilitate the identification of Echinococcus factors involved in such processes.}, added-at = {2011-04-07T15:44:20.000+0200}, author = {Gelmedin, Verena and {Zavala-Góngora}, Ricardo and Fernández, Cecilia and Brehm, Klaus}, biburl = {https://www.bibsonomy.org/bibtex/25838446ac9d563259312a10258172e85/hymi}, doi = {10.1016/j.exppara.2005.04.004}, interhash = {a1385687481352f3862832602bafb8b8}, intrahash = {5838446ac9d563259312a10258172e85}, issn = {0014-4894}, journal = {Experimental Parasitology}, keywords = {Acid Acid, Alignment, Amino Animals, Base Chain Cloning, Complementary, Data, Echinococcus Helminth, Homology, Molecular Molecular, Nuclear Polymerase Proteins, Reaction, Reverse Sequence Sequence, Transcriptase ag_brehm multilocularis, {DNA,} {Trans-Activators}}, month = oct, note = {{PMID:} 15936017}, number = 2, pages = {115--120}, shorttitle = {Echinococcus multilocularis}, timestamp = {2011-04-07T16:41:09.000+0200}, title = {Echinococcus multilocularis: cloning and characterization of a member of the {SNW/SKIP} family of transcriptional coregulators}, url = {http://www.ncbi.nlm.nih.gov/pubmed/15936017}, volume = 111, year = 2005 }