%0 Journal Article %1 Chen_1993_740 %A Cheng, H. %A Lederer, W. J. %A Cannell, M. B. %D 1993 %J Science %K 8235594 Aniline Animals, Anura, Biological, Buffers, Calcium Calcium, Channel Channel, Channels, Compounds, Contraction, Dyes, Electric Electrophysiology, Factors, Fluorescence, Fluorescent Gap Gating, Gov't, In Ion Junctions, Kinetics, Microscopy, Models, Muscle Muscle, Myocardial Myocardium, Myofibrils, Non-U.S. P.H.S., Proteins, Rats, Receptor Release Research Reticulum, Rya, Ryanodine, Sarcoplasmic Skeletal, Stimulation, Support, Time U.S. Vitro, Xanthenes, nodine %N 5134 %P 740--744 %T Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle. %V 262 %X Spontaneous local increases in the concentration of intracellular calcium, called "calcium sparks," were detected in quiescent rat heart cells with a laser scanning confocal microscope and the fluorescent calcium indicator fluo-3. Estimates of calcium flux associated with the sparks suggest that calcium sparks result from spontaneous openings of single sarcoplasmic reticulum (SR) calcium-release channels, a finding supported by ryanodine-dependent changes of spark kinetics. At resting intracellular calcium concentrations, these SR calcium-release channels had a low rate of opening (approximately 0.0001 per second). An increase in the calcium content of the SR, however, was associated with a fourfold increase in opening rate and resulted in some sparks triggering propagating waves of increased intracellular calcium concentration. The calcium spark is the consequence of elementary events underlying excitation-contraction coupling and provides an explanation for both spontaneous and triggered changes in the intracellular calcium concentration in the mammalian heart.

@article{Chen_1993_740, abstract = {Spontaneous local increases in the concentration of intracellular calcium, called "calcium sparks," were detected in quiescent rat heart cells with a laser scanning confocal microscope and the fluorescent calcium indicator fluo-3. Estimates of calcium flux associated with the sparks suggest that calcium sparks result from spontaneous openings of single sarcoplasmic reticulum (SR) calcium-release channels, a finding supported by ryanodine-dependent changes of spark kinetics. At resting intracellular calcium concentrations, these SR calcium-release channels had a low rate of opening (approximately 0.0001 per second). An increase in the calcium content of the SR, however, was associated with a fourfold increase in opening rate and resulted in some sparks triggering propagating waves of increased intracellular calcium concentration. The calcium spark is the consequence of elementary events underlying excitation-contraction coupling and provides an explanation for both spontaneous and triggered changes in the intracellular calcium concentration in the mammalian heart.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Cheng, H. and Lederer, W. J. and Cannell, M. B.}, biburl = {https://www.bibsonomy.org/bibtex/27dd532ffe53739f34b3a4ccb26dd049c/hake}, description = {The whole bibliography file I use.}, file = {Chen_1993_740.pdf:Chen_1993_740.pdf:PDF}, interhash = {a4c1e5e01810c91b89c0883dd2a3dd31}, intrahash = {7dd532ffe53739f34b3a4ccb26dd049c}, journal = {Science}, key = 155, keywords = {8235594 Aniline Animals, Anura, Biological, Buffers, Calcium Calcium, Channel Channel, Channels, Compounds, Contraction, Dyes, Electric Electrophysiology, Factors, Fluorescence, Fluorescent Gap Gating, Gov't, In Ion Junctions, Kinetics, Microscopy, Models, Muscle Muscle, Myocardial Myocardium, Myofibrils, Non-U.S. P.H.S., Proteins, Rats, Receptor Release Research Reticulum, Rya, Ryanodine, Sarcoplasmic Skeletal, Stimulation, Support, Time U.S. Vitro, Xanthenes, nodine}, month = Oct, number = 5134, pages = {740--744}, pdf = {Chen_1993_740.pdf}, pmid = {8235594}, timestamp = {2009-06-03T11:21:08.000+0200}, title = {Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle.}, volume = 262, year = 1993 }