%0 Journal Article %1 wan2019comparing %A Wan, W. L. %A Zhang, L. %A Pruitt, R. %A Zaidem, M. %A Brugman, R. %A Ma, X. %A Krol, E. %A Perraki, A. %A Kilian, J. %A Grossmann, G. %A Stahl, M. %A Shan, L. %A Zipfel, C. %A van Kan, J. A. L. %A Hedrich, R. %A Weigel, D. %A Gust, A. A. %A Nurnberger, T. %D 2019 %J New Phytol %K Arabidopsis Proteins/*metabolism myOwn %N 4 %P 2080-2095 %R 10.1111/nph.15497 %T Comparing Arabidopsis receptor kinase and receptor protein-mediated immune signaling reveals BIK1-dependent differences %U https://www.ncbi.nlm.nih.gov/pubmed/30252144 %V 221 %X Pattern recognition receptors (PRRs) sense microbial patterns and activate innate immunity against attempted microbial invasions. The leucine-rich repeat receptor kinases (LRR-RK) FLS2 and EFR, and the LRR receptor protein (LRR-RP) receptors RLP23 and RLP42, respectively, represent prototypical members of these two prominent and closely related PRR families. We conducted a survey of Arabidopsis thaliana immune signaling mediated by these receptors to address the question of commonalities and differences between LRR-RK and LRR-RP signaling. Quantitative differences in timing and amplitude were observed for several early immune responses, with RP-mediated responses typically being slower and more prolonged than those mediated by RKs. Activation of RLP23, but not FLS2, induced the production of camalexin. Transcriptomic analysis revealed that RLP23-regulated genes represent only a fraction of those genes differentially expressed upon FLS2 activation. Several positive and negative regulators of FLS2-signaling play similar roles in RLP23 signaling. Intriguingly, the cytoplasmic receptor kinase BIK1, a positive regulator of RK signaling, acts as a negative regulator of RP-type immune receptors in a manner dependent on BIK1 kinase activity. Our study unveiled unexpected differences in two closely related receptor systems and reports a new negative role of BIK1 in plant immunity.

@article{wan2019comparing, abstract = {Pattern recognition receptors (PRRs) sense microbial patterns and activate innate immunity against attempted microbial invasions. The leucine-rich repeat receptor kinases (LRR-RK) FLS2 and EFR, and the LRR receptor protein (LRR-RP) receptors RLP23 and RLP42, respectively, represent prototypical members of these two prominent and closely related PRR families. We conducted a survey of Arabidopsis thaliana immune signaling mediated by these receptors to address the question of commonalities and differences between LRR-RK and LRR-RP signaling. Quantitative differences in timing and amplitude were observed for several early immune responses, with RP-mediated responses typically being slower and more prolonged than those mediated by RKs. Activation of RLP23, but not FLS2, induced the production of camalexin. Transcriptomic analysis revealed that RLP23-regulated genes represent only a fraction of those genes differentially expressed upon FLS2 activation. Several positive and negative regulators of FLS2-signaling play similar roles in RLP23 signaling. Intriguingly, the cytoplasmic receptor kinase BIK1, a positive regulator of RK signaling, acts as a negative regulator of RP-type immune receptors in a manner dependent on BIK1 kinase activity. Our study unveiled unexpected differences in two closely related receptor systems and reports a new negative role of BIK1 in plant immunity.}, added-at = {2024-02-15T15:08:22.000+0100}, author = {Wan, W. L. and Zhang, L. and Pruitt, R. and Zaidem, M. and Brugman, R. and Ma, X. and Krol, E. and Perraki, A. and Kilian, J. and Grossmann, G. and Stahl, M. and Shan, L. and Zipfel, C. and van Kan, J. A. L. and Hedrich, R. and Weigel, D. and Gust, A. A. and Nurnberger, T.}, biburl = {https://www.bibsonomy.org/bibtex/293b67e3ca967f00da33b50a6352d2036/jvsi_all}, doi = {10.1111/nph.15497}, interhash = {a9b3a98fb6ccde0c90a5dcdff3eaf3c0}, intrahash = {93b67e3ca967f00da33b50a6352d2036}, issn = {1469-8137 (Electronic) 0028-646X (Print) 0028-646X (Linking)}, journal = {New Phytol}, keywords = {Arabidopsis Proteins/*metabolism myOwn}, note = {Wan, Wei-Lin Zhang, Lisha Pruitt, Rory Zaidem, Maricris Brugman, Rik Ma, Xiyu Krol, Elzbieta Perraki, Artemis Kilian, Joachim Grossmann, Guido Stahl, Mark Shan, Libo Zipfel, Cyril van Kan, Jan A L Hedrich, Rainer Weigel, Detlef Gust, Andrea A Nurnberger, Thorsten eng R01GM097247/NHI/International Nu70/15-1/Deutsche Forschungsgemeinschaft/International A-1795/Robert A. Welch foundation/International Gu1034/3-1/Deutsche Forschungsgemeinschaft/International Gatsby Charitable Foundation/International Cell Networks research group funds/International SFB1101/Deutsche Forschungsgemeinschaft/International Frontier grant from Heidelberg University/International Max-Planck-Gesellschaft/International R01 GM097247/GM/NIGMS NIH HHS/ ERC_/European Research Council/International Comparative Study Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2018/09/27 New Phytol. 2019 Mar;221(4):2080-2095. doi: 10.1111/nph.15497. Epub 2018 Oct 25.}, number = 4, pages = {2080-2095}, timestamp = {2024-02-15T15:08:22.000+0100}, title = {Comparing Arabidopsis receptor kinase and receptor protein-mediated immune signaling reveals BIK1-dependent differences}, type = {Journal Article}, url = {https://www.ncbi.nlm.nih.gov/pubmed/30252144}, volume = 221, year = 2019 }