%0 Journal Article %1 Lee.2008b %A Lee, H. M. %A Kim, H. J. %A Won, K. J. %A Choi, W. S. %A Park, S. H. %A Song, H. %A Park, P. J. %A Park, T. K. %A Lee, C. K. %A Kim, B. %D 2008 %J J.Vasc.Res. %K & Adhesion Animals Aorta Cell Chemistry Cultured Dose-Response Drug Growth Hypertension Inbred Male Molecule-1 Movement Muscle Myocytes Phosphorylation Proliferation Rats Relationship Research SHR Signal Smooth Solubility Sprague-Dawley Transduction Tyrosine Vascular WKY administration cells cytology development dosage drug effects growth methods pathology pharmacology physiopathology response %N 3 %P 259-268 %T Soluble form of vascular cell adhesion molecule 1 induces migration and proliferation of vascular smooth muscle cells %U PM:18182825 %V 45 %X BACKGROUND: Serum levels of soluble vascular cell adhesion molecule 1 (sVCAM-1) shed from its membrane-bound form are elevated in hypertension. This study clarified the effects of sVCAM-1 on vascular responses in rat aortic smooth muscle cells (RASMCs). METHODS: Boyden chamber, 5-bromo-2'-deoxyuridine incorporation and ex vivo aortic ring assays for migration and proliferation, and Western blot for the kinase activity were used. RESULTS: Spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats were compared functionally. sVCAM-1 increased RASMC migration and proliferation, which were greater in SHR compared with WKY rats. RASMCs expressed the very late antigen 4alpha receptor integrin with no difference between SHR and WKY rats. Inhibitors of phosphoinositide kinase 3 (PI3K) and spleen tyrosine kinase (Syk) and small interference RNA-Syk abolished the sVCAM-1-induced migration, proliferation and phosphorylation of focal adhesion kinase. The phosphorylation of Syk was significa

@article{Lee.2008b, abstract = {BACKGROUND: Serum levels of soluble vascular cell adhesion molecule 1 (sVCAM-1) shed from its membrane-bound form are elevated in hypertension. This study clarified the effects of sVCAM-1 on vascular responses in rat aortic smooth muscle cells (RASMCs). METHODS: Boyden chamber, 5-bromo-2'-deoxyuridine incorporation and ex vivo aortic ring assays for migration and proliferation, and Western blot for the kinase activity were used. RESULTS: Spontaneously hypertensive rats (SHR) and Wistar Kyoto (WKY) rats were compared functionally. sVCAM-1 increased RASMC migration and proliferation, which were greater in SHR compared with WKY rats. RASMCs expressed the very late antigen 4alpha receptor integrin with no difference between SHR and WKY rats. Inhibitors of phosphoinositide kinase 3 (PI3K) and spleen tyrosine kinase (Syk) and small interference RNA-Syk abolished the sVCAM-1-induced migration, proliferation and phosphorylation of focal adhesion kinase. The phosphorylation of Syk was significa}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Lee, H. M. and Kim, H. J. and Won, K. J. and Choi, W. S. and Park, S. H. and Song, H. and Park, P. J. and Park, T. K. and Lee, C. K. and Kim, B.}, biburl = {https://www.bibsonomy.org/bibtex/211ab4f06439f70ca8ce639c07a74485c/kanefendt}, interhash = {ae410fe3163e686aa3c68d75a90abb74}, intrahash = {11ab4f06439f70ca8ce639c07a74485c}, journal = {J.Vasc.Res.}, keywords = {& Adhesion Animals Aorta Cell Chemistry Cultured Dose-Response Drug Growth Hypertension Inbred Male Molecule-1 Movement Muscle Myocytes Phosphorylation Proliferation Rats Relationship Research SHR Signal Smooth Solubility Sprague-Dawley Transduction Tyrosine Vascular WKY administration cells cytology development dosage drug effects growth methods pathology pharmacology physiopathology response}, number = 3, pages = {259-268}, timestamp = {2010-02-05T11:28:48.000+0100}, title = {Soluble form of vascular cell adhesion molecule 1 induces migration and proliferation of vascular smooth muscle cells}, url = {PM:18182825}, volume = 45, year = 2008 }