%0 Journal Article %1 MiliasArgeitis2011In %A Milias-Argeitis, Andreas %A Summers, Sean %A Stewart-Ornstein, Jacob %A Zuleta, Ignacio %A Pincus, David %A El-Samad, Hana %A Khammash, Mustafa %A Lygeros, John %D 2011 %I Nature Publishing Group %J Nature Biotechnology %K bt3240 gene-expression regulation %N 12 %P 1114--1116 %R 10.1038/nbt.2018 %T In silico feedback for in vivo regulation of a gene expression circuit %U http://dx.doi.org/10.1038/nbt.2018 %V 29 %X We show that difficulties in regulating cellular behavior with synthetic biological circuits may be circumvented using in silico feedback control. By tracking a circuit's output in Saccharomyces cerevisiae in real time, we precisely control its behavior using an in silico feedback algorithm to compute regulatory inputs implemented through a genetically encoded light-responsive module. Moving control functions outside the cell should enable more sophisticated manipulation of cellular processes whenever real-time measurements of cellular variables are possible.

@article{MiliasArgeitis2011In, abstract = { We show that difficulties in regulating cellular behavior with synthetic biological circuits may be circumvented using in silico feedback control. By tracking a circuit's output in Saccharomyces cerevisiae in real time, we precisely control its behavior using an in silico feedback algorithm to compute regulatory inputs implemented through a genetically encoded light-responsive module. Moving control functions outside the cell should enable more sophisticated manipulation of cellular processes whenever real-time measurements of cellular variables are possible. }, added-at = {2018-12-02T16:09:07.000+0100}, author = {Milias-Argeitis, Andreas and Summers, Sean and Stewart-Ornstein, Jacob and Zuleta, Ignacio and Pincus, David and El-Samad, Hana and Khammash, Mustafa and Lygeros, John}, biburl = {https://www.bibsonomy.org/bibtex/239c8906b4d720f951d5e65ba17a07cd8/karthikraman}, citeulike-article-id = {10004716}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nbt.2018}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nbt.2018}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/22057053}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=22057053}, day = 06, doi = {10.1038/nbt.2018}, interhash = {bc010ee5f9f4604c5bd5babd21d231b6}, intrahash = {39c8906b4d720f951d5e65ba17a07cd8}, issn = {1087-0156}, journal = {Nature Biotechnology}, keywords = {bt3240 gene-expression regulation}, month = nov, number = 12, pages = {1114--1116}, pmid = {22057053}, posted-at = {2011-12-13 12:07:59}, priority = {2}, publisher = {Nature Publishing Group}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {In silico feedback for in vivo regulation of a gene expression circuit}, url = {http://dx.doi.org/10.1038/nbt.2018}, volume = 29, year = 2011 }