%0 Journal Article %1 RN1142 %A Dunkel, M. %A Latz, A. %A Schumacher, K. %A Wüller, T. %A Becker, D. %A Hedrich, R. %D 2008 %J Molecular Plant %K fluorescent green myOwn protein %N 6 %P 938-949 %R 10.1093/mp/ssn064 %T Targeting of Vacuolar Membrane Localized Members of the TPK Channel Family %U /brokenurl#<Go to ISI>://WOS:000262858000006 %V 1 %X Four members of the tandem-pore potassium channel family of Arabidopsis thaliana (TPK1, 2, 3, and 5) reside in the vacuolar membrane, whereas TPK4 is a plasma membrane K+-channel. By constructing chimeras between TPK1 and TPK4, we attempted to identify channel domains involved in the trafficking process and found that the TPK1 cytoplasmic C-terminal domain (CT) is critical for the ER- as well as Golgi-sorting steps. Following site-directed mutagenesis, we identified a diacidic motif (IDLE) required for ER-export of TPK1. However, this diacidic motif in the C-terminus is not conserved among other members of the TPK family, and TPK3 sorting is independent of its CT. Moreover, the 14-3-3 binding site of TPK1, essential for channel activation, is not involved in channel sorting.

@article{RN1142, abstract = {Four members of the tandem-pore potassium channel family of Arabidopsis thaliana (TPK1, 2, 3, and 5) reside in the vacuolar membrane, whereas TPK4 is a plasma membrane K+-channel. By constructing chimeras between TPK1 and TPK4, we attempted to identify channel domains involved in the trafficking process and found that the TPK1 cytoplasmic C-terminal domain (CT) is critical for the ER- as well as Golgi-sorting steps. Following site-directed mutagenesis, we identified a diacidic motif (IDLE) required for ER-export of TPK1. However, this diacidic motif in the C-terminus is not conserved among other members of the TPK family, and TPK3 sorting is independent of its CT. Moreover, the 14-3-3 binding site of TPK1, essential for channel activation, is not involved in channel sorting.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Dunkel, M. and Latz, A. and Schumacher, K. and Wüller, T. and Becker, D. and Hedrich, R.}, biburl = {https://www.bibsonomy.org/bibtex/21ff9b22f4f7dda293f36a8ec2896842e/rainerhedrich_2}, doi = {10.1093/mp/ssn064}, interhash = {bd228c2343316358fac57c7a3436fde6}, intrahash = {1ff9b22f4f7dda293f36a8ec2896842e}, issn = {1674-2052}, journal = {Molecular Plant}, keywords = {fluorescent green myOwn protein}, note = {400hg Times Cited:67 Cited References Count:82}, number = 6, pages = {938-949}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {Targeting of Vacuolar Membrane Localized Members of the TPK Channel Family}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000262858000006}, volume = 1, year = 2008 }