%0 Journal Article %1 RN193 %A Garcia-Beltran, O. %A Cassels, B.K. %A Mena, N. %A Nunez, M.T. %A Yanez, O. %A Caballero, J. %D 2014 %I 2013 Elsevier Ltd. %J Tetrahedron Letters %K bilayer, copper, coumarin cu2+-selectivity, derivative, design, dqcauchile dynamics, fluorescent lipid membranes molecular probe, %N 4 %P 873-876 %R 10.1016/j.tetlet.2013.12.033 %T A Coumarinylaldoxime as a Specific Sensor for Cu2+ and Its Biological Application %U /brokenurl#<Go to ISI>://WOS:000330604500023 %V 55 %X In this Letter we present a new probe, (E)-7-(diethylamino)-2-oxo-2H-chromene-3-carbaldehyde oxime (JB), which can detect Cu2+ ions in HEPES buffer under physiological conditions. Benesi-Hildebrand and Job plots demonstrate that the stoichiometry of the Cu2+ complex formed is 2:1. Possible interference with other analytes was examined, and the decrease of the fluorescence of JB at 510 nm when it reacts with Cu2+ was shown to be highly selective. This probe accumulates in the plasmalemma of human neuroblastoma SH-SY5Y cells. Molecular dynamics (MD) simulations revealed thatJB interacts with the lipid bilayer at the level of the glycerol moieties.

@article{RN193, abstract = {In this Letter we present a new probe, (E)-7-(diethylamino)-2-oxo-2H-chromene-3-carbaldehyde oxime (JB), which can detect Cu2+ ions in HEPES buffer under physiological conditions. Benesi-Hildebrand and Job plots demonstrate that the stoichiometry of the Cu2+ complex formed is 2:1. Possible interference with other analytes was examined, and the decrease of the fluorescence of JB at 510 nm when it reacts with Cu2+ was shown to be highly selective. This probe accumulates in the plasmalemma of human neuroblastoma SH-SY5Y cells. Molecular dynamics (MD) simulations revealed thatJB interacts with the lipid bilayer at the level of the glycerol moieties. }, added-at = {2019-12-04T03:57:35.000+0100}, author = {Garcia-Beltran, O. and Cassels, B.K. and Mena, N. and Nunez, M.T. and Yanez, O. and Caballero, J.}, biburl = {https://www.bibsonomy.org/bibtex/27e21c75bcf0a0829309dc659bf6de19a/dqcauchile}, doi = {10.1016/j.tetlet.2013.12.033}, interhash = {bf006eacd2b04b6e7eb5133bcf0022d7}, intrahash = {7e21c75bcf0a0829309dc659bf6de19a}, issn = {0040-4039}, journal = {Tetrahedron Letters}, keywords = {bilayer, copper, coumarin cu2+-selectivity, derivative, design, dqcauchile dynamics, fluorescent lipid membranes molecular probe,}, number = 4, pages = {873-876}, publisher = {2013 Elsevier Ltd.}, timestamp = {2019-12-04T03:58:17.000+0100}, title = {A Coumarinylaldoxime as a Specific Sensor for Cu2+ and Its Biological Application}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000330604500023}, volume = 55, year = 2014 }