%0 Journal Article %1 RN1155 %A Schneider, S. %A Schneidereit, A. %A Konrad, K. R. %A Hajirezaei, M. R. %A Gramann, M. %A Hedrich, R. %A Sauer, N. %D 2006 %J Plant Physiology %K monosaccharide myOwn transporters %N 2 %P 565-577 %R 10.1104/pp.106.077123 %T Arabidopsis INOSITOL TRANSPORTER4 mediates high-affinity H symport of myoinositol across the plasma membrane %U /brokenurl#<Go to ISI>://WOS:000238168800032 %V 141 %X Four genes of the Arabidopsis ( Arabidopsis thaliana) monosaccharide transporter-like superfamily share significant homology with transporter genes previously identified in the common ice plant ( Mesembryanthemum crystallinum), a model system for studies on salt tolerance of higher plants. These ice plant transporters had been discussed as tonoplast proteins catalyzing the inositol-dependent efflux of Na+ ions from vacuoles. The subcellular localization and the physiological role of the homologous proteins in the glycophyte Arabidopsis were unclear. Here we describe Arabidopsis INOSITOL TRANSPORTER4 ( AtINT4), the first member of this subgroup of Arabidopsis monosaccharide transporter-like transporters. Functional analyses of the protein in yeast ( Saccharomyces cerevisiae) and Xenopus laevis oocytes characterize this protein as a highly specific H 1 symporter for myoinositol. These activities and analyses of the subcellular localization of an AtINT4 fusion protein in Arabidopsis and tobacco ( Nicotiana tabacum) reveal that AtINT4 is located in the plasma membrane. AtINT4 promoter-reporter gene plants demonstrate that AtINT4 is strongly expressed in Arabidopsis pollen and phloem companion cells. The potential physiological role of AtINT4 is discussed.

@article{RN1155, abstract = {Four genes of the Arabidopsis ( Arabidopsis thaliana) monosaccharide transporter-like superfamily share significant homology with transporter genes previously identified in the common ice plant ( Mesembryanthemum crystallinum), a model system for studies on salt tolerance of higher plants. These ice plant transporters had been discussed as tonoplast proteins catalyzing the inositol-dependent efflux of Na+ ions from vacuoles. The subcellular localization and the physiological role of the homologous proteins in the glycophyte Arabidopsis were unclear. Here we describe Arabidopsis INOSITOL TRANSPORTER4 ( AtINT4), the first member of this subgroup of Arabidopsis monosaccharide transporter-like transporters. Functional analyses of the protein in yeast ( Saccharomyces cerevisiae) and Xenopus laevis oocytes characterize this protein as a highly specific H 1 symporter for myoinositol. These activities and analyses of the subcellular localization of an AtINT4 fusion protein in Arabidopsis and tobacco ( Nicotiana tabacum) reveal that AtINT4 is located in the plasma membrane. AtINT4 promoter-reporter gene plants demonstrate that AtINT4 is strongly expressed in Arabidopsis pollen and phloem companion cells. The potential physiological role of AtINT4 is discussed.}, added-at = {2024-02-14T14:38:32.000+0100}, author = {Schneider, S. and Schneidereit, A. and Konrad, K. R. and Hajirezaei, M. R. and Gramann, M. and Hedrich, R. and Sauer, N.}, biburl = {https://www.bibsonomy.org/bibtex/2f26cfb2c3f9f8bb483261026d9504640/rainerhedrich_2}, doi = {10.1104/pp.106.077123}, interhash = {bf1223545aebab02a7f61e666800b52c}, intrahash = {f26cfb2c3f9f8bb483261026d9504640}, issn = {0032-0889}, journal = {Plant Physiology}, keywords = {monosaccharide myOwn transporters}, note = {051nw Times Cited:62 Cited References Count:69}, number = 2, pages = {565-577}, timestamp = {2024-02-14T14:38:32.000+0100}, title = {Arabidopsis INOSITOL TRANSPORTER4 mediates high-affinity H symport of myoinositol across the plasma membrane}, type = {Journal Article}, url = {/brokenurl#<Go to ISI>://WOS:000238168800032}, volume = 141, year = 2006 }