%0 Journal Article %1 Kuzmina2011 %A Kuzmina, Zoya %A Greinix, Hildegard T. %A Weigl, Roman %A Körmöczi, Ulrike %A Rottal, Arno %A Frantal, Sophie %A Eder, Sandra %A Pickl, Winfried F. %D 2011 %J Blood %K bcells Transplantation antigens balf facs mucosal %N 7 %P 2265--2274 %R 10.1182/blood-2010-07-295766 %T Significant differences in B-cell subpopulations characterize patients with chronic graft-versus-host disease-associated dysgammaglobulinemia. %U http://dx.doi.org/10.1182/blood-2010-07-295766 %V 117 %X Manifestations of chronic graft-versus-host disease (cGVHD) can resemble those seen in immunodeficiency states and autoimmune disorders. Reports by us and others suggest an involvement of B cells in the pathogenesis of cGVHD. We investigated B-lymphocyte subpopulations in cGVHD cohorts defined by serum immunoglobulin G (IgG) levels to characterize novel biomarkers for impairment of humoral immunity after allogeneic hematopoietic stem cell transplantation. Seventy-six patients were enrolled a median of 46 months after hematopoietic stem cell transplantation. The hypogammaglobulinemia group had significantly diminished CD19(+) B cells (165 vs 454 vs 417 × 10⁶L) with elevated CD19(+)CD21(low) immature (16.5\%, 7.7\%, and 9.1\%) and CD19(+)CD21(int-high)CD38(high)IgM(high) transitional (10.5\% vs 4.2\% vs 6.3\%) B-cell proportions compared with the normogammaglobulinemia and hypergammaglobulinemia groups. CD19(+)CD10(-)CD27(-)CD21(high) naive B cells were highly elevated in all patients with cGVHD. CD19(+)CD27(+)IgD(+) non-class-switched (4 vs 12 vs 11 × 10⁶/L) and class-switched (7 vs 35 vs 42 × 10⁶/L) memory B cells were significantly lower in the hypogammaglobulinemia group compared with the others. Besides significantly higher B-cell activation factor/B-cell ratios, significantly more cGVHD patients with hypergammaglobulinemia had autoantibodies compared with the hypogammaglobulinemia subgroup (68\% vs 24\%, P = .024). In conclusion, B-cell subpopulations can serve as novel cellular biomarkers for immunodeficiency and autoimmunity indicating different pathogenetic mechanisms of cGVHD and encouraging future prospective longitudinal studies.

@article{Kuzmina2011, abstract = {Manifestations of chronic graft-versus-host disease (cGVHD) can resemble those seen in immunodeficiency states and autoimmune disorders. Reports by us and others suggest an involvement of B cells in the pathogenesis of cGVHD. We investigated B-lymphocyte subpopulations in cGVHD cohorts defined by serum immunoglobulin G (IgG) levels to characterize novel biomarkers for impairment of humoral immunity after allogeneic hematopoietic stem cell transplantation. Seventy-six patients were enrolled a median of 46 months after hematopoietic stem cell transplantation. The hypogammaglobulinemia group had significantly diminished CD19(+) B cells (165 vs 454 vs 417 × 10⁶L) with elevated CD19(+)CD21(low) immature (16.5\%, 7.7\%, and 9.1\%) and CD19(+)CD21(int-high)CD38(high)IgM(high) transitional (10.5\% vs 4.2\% vs 6.3\%) B-cell proportions compared with the normogammaglobulinemia and hypergammaglobulinemia groups. CD19(+)CD10(-)CD27(-)CD21(high) naive B cells were highly elevated in all patients with cGVHD. CD19(+)CD27(+)IgD(+) non-class-switched (4 vs 12 vs 11 × 10⁶/L) and class-switched (7 vs 35 vs 42 × 10⁶/L) memory B cells were significantly lower in the hypogammaglobulinemia group compared with the others. Besides significantly higher B-cell activation factor/B-cell ratios, significantly more cGVHD patients with hypergammaglobulinemia had autoantibodies compared with the hypogammaglobulinemia subgroup (68\% vs 24\%, P = .024). In conclusion, B-cell subpopulations can serve as novel cellular biomarkers for immunodeficiency and autoimmunity indicating different pathogenetic mechanisms of cGVHD and encouraging future prospective longitudinal studies.}, added-at = {2013-03-21T00:44:10.000+0100}, author = {Kuzmina, Zoya and Greinix, Hildegard T. and Weigl, Roman and Körmöczi, Ulrike and Rottal, Arno and Frantal, Sophie and Eder, Sandra and Pickl, Winfried F.}, biburl = {https://www.bibsonomy.org/bibtex/2c1faa4a764936044be152885f8e2fb07/aorchid}, description = {see page 86 notebook 00002, Aki keeps refering to this one}, doi = {10.1182/blood-2010-07-295766}, file = {:allorejection/bcell/Blood.117.2265.pdf:PDF}, groups = {public}, institution = {Department of Internal Medicine I, Bone Marrow Transplantation, Medical University of Vienna, Vienna, Austria.}, interhash = {c5db221b114a9333fb0bb0a2acf10f83}, intrahash = {c1faa4a764936044be152885f8e2fb07}, journal = {Blood}, keywords = {bcells Transplantation antigens balf facs mucosal}, language = {eng}, medline-pst = {ppublish}, month = Feb, number = 7, pages = {2265--2274}, pii = {blood-2010-07-295766}, pmid = {21063025}, timestamp = {2013-03-21T00:44:10.000+0100}, title = {Significant differences in B-cell subpopulations characterize patients with chronic graft-versus-host disease-associated dysgammaglobulinemia.}, url = {http://dx.doi.org/10.1182/blood-2010-07-295766}, username = {aorchid}, volume = 117, year = 2011 }