%0 Journal Article %1 citeulike:693531 %A Trau, D. %A Theueri, T. %A Wilmer, M. %A Meusel, M. %A Spener, F. %C Institute of Chemical and Biochemical Sensor Research, ICB, Münster, Germany. %D 1997 %J Biosens Bioelectron %K amperometry a-p immunoassay electrochemistry %N 6 %P 499--510 %T Development of an amperometric flow injection immunoanalysis system for the determination of the herbicide 2,4-dichlorophenoxyacetic acid in water. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9253154 %V 12 %X An amperometric flow injection immunoanalysis (FIIA) system based on an immunoreactor with immobilized biocomponents on a silica surface has been developed for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). In the antigen coating mode the hapten was immobilized and monoclonal primary antibody against 2,4-D together with alkaline phosphatase (AP)-labelled secondary antibody were used as sensing elements in a titration assay. In the antibody coating mode a biotinylated monoclonal antibody was immobilized on the surface of the immunoreactor and a 2,4-D-AP-conjugate was used for detection. For electrochemical measurements p-aminophenol enzymatically generated from p-aminophenyl phosphate was oxidized at a carbon working electrode at +150 mV versus Ag/AgCl. The system enabled the determination of 2,4-D in drinking water samples in the range from 0.2 to 70 micrograms/l. The whole system was computer controlled with a measuring time of 12 min for one determination.

@article{citeulike:693531, abstract = {An amperometric flow injection immunoanalysis (FIIA) system based on an immunoreactor with immobilized biocomponents on a silica surface has been developed for the determination of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). In the antigen coating mode the hapten was immobilized and monoclonal primary antibody against 2,4-D together with alkaline phosphatase (AP)-labelled secondary antibody were used as sensing elements in a titration assay. In the antibody coating mode a biotinylated monoclonal antibody was immobilized on the surface of the immunoreactor and a 2,4-D-AP-conjugate was used for detection. For electrochemical measurements p-aminophenol enzymatically generated from p-aminophenyl phosphate was oxidized at a carbon working electrode at +150 mV versus Ag/AgCl. The system enabled the determination of 2,4-D in drinking water samples in the range from 0.2 to 70 micrograms/l. The whole system was computer controlled with a measuring time of 12 min for one determination.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Institute of Chemical and Biochemical Sensor Research, ICB, Münster, Germany.}, author = {Trau, D. and Theueri, T. and Wilmer, M. and Meusel, M. and Spener, F.}, biburl = {https://www.bibsonomy.org/bibtex/2303bbe7cc308c27ef964241cbe174998/biblio24}, citeulike-article-id = {693531}, interhash = {c657f6681964dc299d1cc6c0ef51c050}, intrahash = {303bbe7cc308c27ef964241cbe174998}, issn = {0956-5663}, journal = {Biosens Bioelectron}, keywords = {amperometry a-p immunoassay electrochemistry}, number = 6, pages = {499--510}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Development of an amperometric flow injection immunoanalysis system for the determination of the herbicide 2,4-dichlorophenoxyacetic acid in water.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=9253154}, volume = 12, year = 1997 }