%0 Journal Article %1 RefWorks:2406 %A Hodge, G. %A Hodge, S. %A Reynolds, P. N. %A Holmes, M. %D 2008 %J Transpl Infect Dis %K facs transplantation balf bos pseudomonas staph virus %N 2 %P 99--105 %R 10.1111/j.1399-3062.2007.00236.x %T Airway infection in stable lung transplant patients is associated with decreased intracellular T-helper type 1 pro-inflammatory cytokines in bronchoalveolar lavage T-cell subsets. %U http://dx.doi.org/10.1111/j.1399-3062.2007.00236.x %V 10 %X Current immunosuppression protocols to prevent lung transplant rejection reduce pro-inflammatory and T-helper type 1 (Th1) cytokines. However, Th1 T-cell pro-inflammatory cytokine production is important in host defense against bacterial infection in the lungs. Excessive immunosuppression of Th1 T-cell pro-inflammatory cytokines leaves patients susceptible to infection. To investigate whether pulmonary infection in lung transplant recipients is associated with reduced Th1 T-cell pro-inflammatory cytokines, whole blood and bronchoalveolar lavage (BAL) fluid from 13 stable lung transplant patients with 'culture-negative' BAL and 13 patients with 'culture-positive' BAL was stimulated in vitro, and cytokine production by CD8+ and CD4+ T-cell subsets was determined using multiparameter flow cytometry. In BAL samples, there was a significant decrease in interleukin-2 (IL2) in CD3+ T cells and tumor necrosis factor-alpha (TNF-alpha) in CD8+ T cells (but not CD4+) in 'culture-positive' compared with 'culture-negative' transplant patients. There was no difference in blood Th1 T-cell cytokines between 'culture-positive' compared with 'culture-negative' transplant patients. A decrease in Th1 cytokines IL-2 and TNF-alpha in BAL T-cell subsets is associated with isolation of potentially pathogenic organisms in the lungs in stable lung transplant patients. Excessive immunosuppression of these Th1 T-cell pro-inflammatory cytokines in stable transplant patients may leave them susceptible to infection. Modifying immunosuppression by monitoring intracellular Th1 pro-inflammatory cytokines in BAL T cells may help to improve morbidity and infection rates in stable lung transplant patients.

@article{RefWorks:2406, abstract = {Current immunosuppression protocols to prevent lung transplant rejection reduce pro-inflammatory and T-helper type 1 (Th1) cytokines. However, Th1 T-cell pro-inflammatory cytokine production is important in host defense against bacterial infection in the lungs. Excessive immunosuppression of Th1 T-cell pro-inflammatory cytokines leaves patients susceptible to infection. To investigate whether pulmonary infection in lung transplant recipients is associated with reduced Th1 T-cell pro-inflammatory cytokines, whole blood and bronchoalveolar lavage (BAL) fluid from 13 stable lung transplant patients with 'culture-negative' BAL and 13 patients with 'culture-positive' BAL was stimulated in vitro, and cytokine production by CD8+ and CD4+ T-cell subsets was determined using multiparameter flow cytometry. In BAL samples, there was a significant decrease in interleukin-2 (IL2) in CD3+ T cells and tumor necrosis factor-alpha (TNF-alpha) in CD8+ T cells (but not CD4+) in 'culture-positive' compared with 'culture-negative' transplant patients. There was no difference in blood Th1 T-cell cytokines between 'culture-positive' compared with 'culture-negative' transplant patients. A decrease in Th1 cytokines IL-2 and TNF-alpha in BAL T-cell subsets is associated with isolation of potentially pathogenic organisms in the lungs in stable lung transplant patients. Excessive immunosuppression of these Th1 T-cell pro-inflammatory cytokines in stable transplant patients may leave them susceptible to infection. Modifying immunosuppression by monitoring intracellular Th1 pro-inflammatory cytokines in BAL T cells may help to improve morbidity and infection rates in stable lung transplant patients.}, added-at = {2012-12-08T03:55:43.000+0100}, author = {Hodge, G. and Hodge, S. and Reynolds, P. N. and Holmes, M.}, biburl = {https://www.bibsonomy.org/bibtex/22b5c40e9749dc59a6082cb61b1ec760d/aorchid}, doi = {10.1111/j.1399-3062.2007.00236.x}, file = {:allorejection/TransplInfectDis.10.99.pdf:PDF}, groups = {public}, institution = {Department of Thoracic Medicine, Royal Adelaide Hospital, Adelaide, South Australia, Australia. greg.hodge@cywhs.sa.gov.au}, interhash = {c73bf451ef36aa1813393eabbb9c779a}, intrahash = {2b5c40e9749dc59a6082cb61b1ec760d}, journal = {Transpl Infect Dis}, keywords = {facs transplantation balf bos pseudomonas staph virus}, language = {eng}, medline-pst = {ppublish}, month = Apr, number = 2, pages = {99--105}, pii = {TID236}, pmid = {17511818}, timestamp = {2012-12-08T03:55:43.000+0100}, title = {Airway infection in stable lung transplant patients is associated with decreased intracellular T-helper type 1 pro-inflammatory cytokines in bronchoalveolar lavage T-cell subsets.}, url = {http://dx.doi.org/10.1111/j.1399-3062.2007.00236.x}, username = {aorchid}, volume = 10, year = 2008 }