%0 Journal Article %1 Husain2010 %A Husain, Shahid %A Capitano, Blair %A Corcoran, Timothy %A Studer, Sean M. %A Crespo, Maria %A Johnson, Bruce %A Pilewski, Joseph M. %A Shutt, Kathleen %A Pakstis, Diana L. %A Zhang, Shimin %A Carey, Mary Ellen %A Paterson, David L. %A McCurry, Kenneth R. %A Venkataramanan, Raman %D 2010 %J Transplantation %K drug dosing mucosal fungal transplantation %N 11 %P 1215--1219 %R 10.1097/TP.0b013e3181f995ea %T Intrapulmonary disposition of amphotericin B after aerosolized delivery of amphotericin B lipid complex (Abelcet; ABLC) in lung transplant recipients. %U http://dx.doi.org/10.1097/TP.0b013e3181f995ea %V 90 %X Inhaled amphotericin preparations have been used for prophylaxis against invasive aspergillosis in lung transplant recipients. However, no published data exist regarding the pharmacokinetic profile of amphotericin B lipid complex in lung transplant recipients.We prospectively determined the concentrations of amphotericin B in the epithelial lining fluid (ELF) and plasma after aerosolized nebulization (AeroEclipse), of amphotericin B lipid complex at 1 mg/kg every 24 hr for 4 days in 35 lung transplant recipients. One brochoalveolar lavage sample and a simultaneous blood sample were collected at various time points after the fourth dose from each subject. High-performance liquid chromatography and high-performance liquid chromatography-MS-MS were used to measure amphotericin B.Concentrations of amphotericin B in ELF (median, 25-75 IQR) were at 4 hr (n=5) 7.20 μg/mL (1.3-17.6), 24 hr (n=6) 8.26 μg/mL (3.9-82.7), 48 hr (n=5) 2.15 μg/mL (1.4-5.5), 72 hr (n=4) 1.25 μg/mL (0.75-5.5), 96 hr (n=6) 0.86 μg/mL (0.55-1.4), 120 hr (n=4) 1.04 μg/mL (0.44-1.6), 144 hr (n=1), 4.25 μg/mL, 168 hr (n=3) 1.14 μg/mL, and 192 hr (n=1) 1 μg/mL. The plasma concentration of the drug remained below 0.08 μg/mL at all time points. During the study, the side effects noted included wheezing, coughing, and 12\% decline in forced expiratory volume in 1 sec.We conclude that administration through aerosolized nebulization of amphotericin B lipid complex every 24 hr for 4 days in lung transplant recipients achieved amphotericin B concentrations in ELF above minimum inhibitory concentration of the Aspergillus nearly at 168 hr after the last inhaled dose and is well tolerated.

@article{Husain2010, abstract = {Inhaled amphotericin preparations have been used for prophylaxis against invasive aspergillosis in lung transplant recipients. However, no published data exist regarding the pharmacokinetic profile of amphotericin B lipid complex in lung transplant recipients.We prospectively determined the concentrations of amphotericin B in the epithelial lining fluid (ELF) and plasma after aerosolized nebulization (AeroEclipse), of amphotericin B lipid complex at 1 mg/kg every 24 hr for 4 days in 35 lung transplant recipients. One brochoalveolar lavage sample and a simultaneous blood sample were collected at various time points after the fourth dose from each subject. High-performance liquid chromatography and high-performance liquid chromatography-MS-MS were used to measure amphotericin B.Concentrations of amphotericin B in ELF (median, 25-75 IQR) were at 4 hr (n=5) 7.20 μg/mL (1.3-17.6), 24 hr (n=6) 8.26 μg/mL (3.9-82.7), 48 hr (n=5) 2.15 μg/mL (1.4-5.5), 72 hr (n=4) 1.25 μg/mL (0.75-5.5), 96 hr (n=6) 0.86 μg/mL (0.55-1.4), 120 hr (n=4) 1.04 μg/mL (0.44-1.6), 144 hr (n=1), 4.25 μg/mL, 168 hr (n=3) 1.14 μg/mL, and 192 hr (n=1) 1 μg/mL. The plasma concentration of the drug remained below 0.08 μg/mL at all time points. During the study, the side effects noted included wheezing, coughing, and 12\% decline in forced expiratory volume in 1 sec.We conclude that administration through aerosolized nebulization of amphotericin B lipid complex every 24 hr for 4 days in lung transplant recipients achieved amphotericin B concentrations in ELF above minimum inhibitory concentration of the Aspergillus nearly at 168 hr after the last inhaled dose and is well tolerated.}, added-at = {2013-03-06T20:52:16.000+0100}, author = {Husain, Shahid and Capitano, Blair and Corcoran, Timothy and Studer, Sean M. and Crespo, Maria and Johnson, Bruce and Pilewski, Joseph M. and Shutt, Kathleen and Pakstis, Diana L. and Zhang, Shimin and Carey, Mary Ellen and Paterson, David L. and McCurry, Kenneth R. and Venkataramanan, Raman}, biburl = {https://www.bibsonomy.org/bibtex/2a5a2e0c043a72b6da12857e69280e21b/aorchid}, description = {concentrations of inhaled ABLC in the BAL were good. 35 patients samples drawn over 4 days of daily ABLC inhaled. Used a measure of epithelial lining fluid based on the concentration of urea in the BAL to baseline them all.}, doi = {10.1097/TP.0b013e3181f995ea}, file = {:ID_General/Fungus_most/Transplantation.90.1215.pdf:PDF}, groups = {public}, institution = {Division of Infectious Diseases, University of Pittsburgh, Pittsburgh, PA, USA. shahid.husain@uhn.on.ca}, interhash = {c85f80422c88be1bbd00db4ae4d22569}, intrahash = {a5a2e0c043a72b6da12857e69280e21b}, journal = {Transplantation}, keywords = {drug dosing mucosal fungal transplantation}, language = {eng}, medline-pst = {ppublish}, month = Dec, number = 11, pages = {1215--1219}, pmid = {20881664}, timestamp = {2013-03-06T20:52:16.000+0100}, title = {Intrapulmonary disposition of amphotericin B after aerosolized delivery of amphotericin B lipid complex (Abelcet; ABLC) in lung transplant recipients.}, url = {http://dx.doi.org/10.1097/TP.0b013e3181f995ea}, username = {aorchid}, volume = 90, year = 2010 }