%0 Journal Article %1 chamberland_modulation_2010 %A Chamberland, Caroline %A Barajas-Martinez, Hector %A Haufe, Volker %A Fecteau, Marie-Hélène %A Delabre, Jean-Francois %A Burashnikov, Alexander %A Antzelevitch, Charles %A Lesur, Olivier %A Chraibi, Ahmed %A Sarret, Philippe %A Dumaine, Robert %D 2010 %J Journal of Molecular and Cellular Cardiology %K Action_Potentials Animals Carrier_Proteins Dogs Electrophysiology Heart Humans Immunohistochemistry Mice Myocardium Pericardium Rats Sodium Sodium_Channels {Patch-Clamp}_Techniques %N 4 %P 694--701 %R 10.1016/j.yjmcc.2009.12.011 %T Modulation of canine cardiac sodium current by Apelin %U http://www.ncbi.nlm.nih.gov/pubmed/20036246 %V 48 %X Apelin, a ligand of the G protein-coupled putative angiotensin II-like receptor (APJ-R), exerts strong vasodilating, cardiac inotropic and chronotropic actions. Its expression is highly up-regulated during heart failure. Apelin also increases cardiac conduction speed and excitability. While our knowledge of apelin cardiovascular actions is growing, our understanding of the physiological mechanisms behind the cardiac effects remains limited. We tested the effects of apelin on the cardiac sodium current (I(Na)) using patch clamp technique on cardiac myocytes acutely dissociated from dog ventricle. We found that apelin-13 and apelin-17 increased peak I(Na) by 39\% and 61\% and shifted its mid-activation potential by -6.8+/-0.6 mV and -17+/-1 mV respectively thus increasing channel opening at negative voltage. Apelin also slowed I(Na) recovery from inactivation. The effects of apelin on I(Na) amplitude were linked to activation of protein kinase C. Apelin also increased I(Na) "window" current by up to 600\% suggesting that changes in intracellular sodium may contribute to the apelin inotropic effects. Our results reveal for the first time the effects of apelin on I(Na). These effects are likely to modulate cardiac conduction and excitability and may have beneficial antiarrhythmic action in sodium chanelopathies such as Brugada Syndrome where I(Na) amplitude is reduced.

@article{chamberland_modulation_2010, abstract = {Apelin, a ligand of the G protein-coupled putative angiotensin {II-like} receptor {(APJ-R)}, exerts strong vasodilating, cardiac inotropic and chronotropic actions. Its expression is highly up-regulated during heart failure. Apelin also increases cardiac conduction speed and excitability. While our knowledge of apelin cardiovascular actions is growing, our understanding of the physiological mechanisms behind the cardiac effects remains limited. We tested the effects of apelin on the cardiac sodium current {(I(Na))} using patch clamp technique on cardiac myocytes acutely dissociated from dog ventricle. We found that apelin-13 and apelin-17 increased peak {I(Na)} by 39\% and 61\% and shifted its mid-activation potential by -6.8+/-0.6 {mV} and -17+/-1 {mV} respectively thus increasing channel opening at negative voltage. Apelin also slowed {I(Na)} recovery from inactivation. The effects of apelin on {I(Na)} amplitude were linked to activation of protein kinase C. Apelin also increased {I(Na)} "window" current by up to 600\% suggesting that changes in intracellular sodium may contribute to the apelin inotropic effects. Our results reveal for the first time the effects of apelin on {I(Na).} These effects are likely to modulate cardiac conduction and excitability and may have beneficial antiarrhythmic action in sodium chanelopathies such as Brugada Syndrome where {I(Na)} amplitude is reduced.}, added-at = {2011-08-03T17:38:07.000+0200}, author = {Chamberland, Caroline and {Barajas-Martinez}, Hector and Haufe, Volker and Fecteau, {Marie-Hélène} and Delabre, {Jean-Francois} and Burashnikov, Alexander and Antzelevitch, Charles and Lesur, Olivier and Chraibi, Ahmed and Sarret, Philippe and Dumaine, Robert}, biburl = {https://www.bibsonomy.org/bibtex/29052affa7feabd42dd2eada1d99250c9/crc_chus}, doi = {10.1016/j.yjmcc.2009.12.011}, interhash = {d08577967ad4c4fe70974d4e804f651a}, intrahash = {9052affa7feabd42dd2eada1d99250c9}, issn = {1095-8584}, journal = {Journal of Molecular and Cellular Cardiology}, keywords = {Action_Potentials Animals Carrier_Proteins Dogs Electrophysiology Heart Humans Immunohistochemistry Mice Myocardium Pericardium Rats Sodium Sodium_Channels {Patch-Clamp}_Techniques}, month = apr, note = {{PMID:} 20036246}, number = 4, pages = {694--701}, timestamp = {2011-08-03T20:51:54.000+0200}, title = {Modulation of canine cardiac sodium current by Apelin}, url = {http://www.ncbi.nlm.nih.gov/pubmed/20036246}, volume = 48, year = 2010 }