%0 Journal Article %1 calatayud_serotype_2010 %A Calatayud, Laura %A Ardanuy, Carmen %A Tubau, Fe %A Rolo, Dora %A Grau, Immaculada %A Pallarés, Román %A Martín, Rogelio %A Liñares, Josefina %D 2010 %J Journal of Clinical Microbiology %K 80 Adolescent, Adult Adult, Aged, Agents, Bacterial Bacterial, Drug Elements, Female, Fingerprinting, Genotype, Humans, Infections, Macrolides, Male, Middle Molecular Pneumococcal Resistance, Serotyping, Spain, Streptococcus Techniques, Transposable Typing Young and epidemiology, over, pneumoniae, {Anti-Bacterial} {DNA} %N 4 %P 1310--1316 %R 10.1128/JCM.01868-09 %T Serotype and genotype replacement among macrolide-resistant invasive Pneumococci in adults: mechanisms of resistance and association with different transposons %U http://www.ncbi.nlm.nih.gov/pubmed/20147647 %V 48 %X The aim of this study was to analyze trends in adult invasive pneumococcal disease (IPD) due to macrolide-resistant strains and to study the evolution of serotypes, genotypes, and macrolide-resistant determinants of strains collected in a prospective study between 1999 and 2007 in Barcelona, Spain. IPD due to macrolide-resistant strains of serotypes included in the 7-valent conjugate vaccine (PCV7) decreased from 2.16/100,000 (pre-PCV7 period, 1999 to 2001) to 0.80/100,000 (late-PCV7 period, 2005 to 2007) (P = 0.001), whereas IPD due to macrolide-resistant strains of non-PCV7 serotypes increased from 1.08/100,000 to 2.83/100,000 (P \textless 0.001). These changes were related to a fall of clones of PCV7 serotypes (ST81 P \textless 0.05, ST90, ST315, and ST17) and an increase in new clones of serotypes 19A and 24F (ST230) and 33F (ST717) in the late-PCV7 period. The most common phenotype was MLS(B) (90.9\%), related to the erm(B) gene. The frequent association between MLS(B) phenotype and tetracycline resistance tet(M) gene, was related to transposons of the Tn916-family such as Tn6002 or Tn3872. In conclusion, overall adult IPD rates due to macrolide-resistant pneumococci stabilized between 1999 and 2007 in Barcelona. The decrease in macrolide-resistant PCV7 pneumococci was balanced by the increase in macrolide-resistant non-PCV7 pneumococci.

@article{calatayud_serotype_2010, abstract = {The aim of this study was to analyze trends in adult invasive pneumococcal disease {(IPD)} due to macrolide-resistant strains and to study the evolution of serotypes, genotypes, and macrolide-resistant determinants of strains collected in a prospective study between 1999 and 2007 in Barcelona, Spain. {IPD} due to macrolide-resistant strains of serotypes included in the 7-valent conjugate vaccine {(PCV7)} decreased from 2.16/100,000 {(pre-PCV7} period, 1999 to 2001) to 0.80/100,000 {(late-PCV7} period, 2005 to 2007) {(P} = 0.001), whereas {IPD} due to macrolide-resistant strains of {non-PCV7} serotypes increased from 1.08/100,000 to 2.83/100,000 {(P} {\textless} 0.001). These changes were related to a fall of clones of {PCV7} serotypes {(ST81} {[P} {\textless} 0.05], {ST90,} {ST315,} and {ST17)} and an increase in new clones of serotypes {19A} and {24F} {(ST230)} and {33F} {(ST717)} in the {late-PCV7} period. The most common phenotype was {MLS(B)} (90.9\%), related to the {erm(B)} gene. The frequent association between {MLS(B)} phenotype and tetracycline resistance {[tet(M)} gene], was related to transposons of the Tn916-family such as Tn6002 or Tn3872. In conclusion, overall adult {IPD} rates due to macrolide-resistant pneumococci stabilized between 1999 and 2007 in Barcelona. The decrease in macrolide-resistant {PCV7} pneumococci was balanced by the increase in macrolide-resistant {non-PCV7} pneumococci.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Calatayud, Laura and Ardanuy, Carmen and Tubau, Fe and Rolo, Dora and Grau, Immaculada and Pallarés, Román and Martín, Rogelio and Liñares, Josefina}, biburl = {https://www.bibsonomy.org/bibtex/21fab6f80a84b4e609ae6d63caf7e7020/jelias}, doi = {10.1128/JCM.01868-09}, interhash = {d26a96ce49a396b009c3e67421b704b9}, intrahash = {1fab6f80a84b4e609ae6d63caf7e7020}, issn = {{1098-660X}}, journal = {Journal of Clinical Microbiology}, keywords = {80 Adolescent, Adult Adult, Aged, Agents, Bacterial Bacterial, Drug Elements, Female, Fingerprinting, Genotype, Humans, Infections, Macrolides, Male, Middle Molecular Pneumococcal Resistance, Serotyping, Spain, Streptococcus Techniques, Transposable Typing Young and epidemiology, over, pneumoniae, {Anti-Bacterial} {DNA}}, month = apr, note = {{PMID:} 20147647}, number = 4, pages = {1310--1316}, shorttitle = {Serotype and genotype replacement among macrolide-resistant invasive Pneumococci in adults}, timestamp = {2011-03-11T10:06:30.000+0100}, title = {Serotype and genotype replacement among macrolide-resistant invasive Pneumococci in adults: mechanisms of resistance and association with different transposons}, url = {http://www.ncbi.nlm.nih.gov/pubmed/20147647}, volume = 48, year = 2010 }