%0 Journal Article %1 Thaete.1995 %A Thaete, C. %A Pongratz, J. %A Koenig, A. %A Klamt, B. %A Tsaoussidou, S. %A Higgins, M. %A Shows, T. %A Jones, C. %A Gessler, M. %D 1995 %J Mamm Genome %K *Chromosomes;Human;Pair 11 Animals Base Cells Chromosome Cloning;Molecular Conserved Cosmids Cricetinae Data Deoxyribonucleases;Type Dinucleoside Humans Hybrid II Mapping Molecular Phosphates/*genetics Sequence Site-Specific %N 6 %P 421--425 %T CpG island clones for chromosome 11p--a resource for mapping and gene identification %V 6 %X A NotI end fragment library has been constructed for human Chromosome (Chr) 11p. Seventy-two clones were mapped to chromosomal subregions by use of somatic cell hybrids. The clones detect 44 different CpG islands, and we have isolated cosmid contigs for 36 of them. Extrapolation from the known 11p13 NotI restriction map suggests that every second CpG island from 11p containing a Not site is already represented in the clone collection. By sequence analysis all of the 11p13 clones exhibit typical features of CpG islands, and cross-species hybridization has been detected with at least one fragment in most cases. The cosmids serve as valuable linking clones for long-range restriction mapping. They also provide excellent starting material for transcript isolation procedures to identify genes on chromosome 11p associated with developmental anomalies and various tumor types. Several transcribed sequences have already been isolated with some of these clones.

@article{Thaete.1995, abstract = {A NotI end fragment library has been constructed for human Chromosome (Chr) 11p. Seventy-two clones were mapped to chromosomal subregions by use of somatic cell hybrids. The clones detect 44 different CpG islands, and we have isolated cosmid contigs for 36 of them. Extrapolation from the known 11p13 NotI restriction map suggests that every second CpG island from 11p containing a Not site is already represented in the clone collection. By sequence analysis all of the 11p13 clones exhibit typical features of CpG islands, and cross-species hybridization has been detected with at least one fragment in most cases. The cosmids serve as valuable linking clones for long-range restriction mapping. They also provide excellent starting material for transcript isolation procedures to identify genes on chromosome 11p associated with developmental anomalies and various tumor types. Several transcribed sequences have already been isolated with some of these clones.}, added-at = {2013-01-29T13:47:26.000+0100}, author = {Thaete, C. and Pongratz, J. and Koenig, A. and Klamt, B. and Tsaoussidou, S. and Higgins, M. and Shows, T. and Jones, C. and Gessler, M.}, biburl = {https://www.bibsonomy.org/bibtex/2360ea9263ffd6ff2dd7bec0ba2a413f7/ebch}, interhash = {d74eabcd50e00e4ec44c3f171d2661af}, intrahash = {360ea9263ffd6ff2dd7bec0ba2a413f7}, journal = {Mamm Genome}, keywords = {*Chromosomes;Human;Pair 11 Animals Base Cells Chromosome Cloning;Molecular Conserved Cosmids Cricetinae Data Deoxyribonucleases;Type Dinucleoside Humans Hybrid II Mapping Molecular Phosphates/*genetics Sequence Site-Specific}, number = 6, pages = {421--425}, timestamp = {2013-01-29T13:47:34.000+0100}, title = {CpG island clones for chromosome 11p--a resource for mapping and gene identification}, volume = 6, year = 1995 }