%0 Journal Article %1 Boss_2002_113 %A Bossuyt, Julie %A Taylor, Bonnie E %A James-Kracke, Marilyn %A Hale, Calvin C %D 2002 %J FEBS Lett. %K 3; Acid Amino Animals; Binding Binding; Blotting, Cattle; Caveolae, Caveolin Caveolins, Centrifugation, Cholesterol, Chromatography, Cyclodextrins, Density Exchanger, Gel; Gradient; Motifs; Myocardium, Precipitin Protein Sarcolemma, Sites; Sodium-Calcium Tests; Western; chemistry/drug chemistry/metabolism effects/metabolism; metabolism; pharmacology; %N 1-3 %P 113--117 %R 10.1016/S0014-5793(01)03323-3 %T Evidence for cardiac sodium-calcium exchanger association with caveolin-3. %U http://dx.doi.org/10.1016/S0014-5793(01)03323-3 %V 511 %X The interaction of cardiac Na$^+$-Ca$^2+$ exchange (NCX1) with caveolin proteins was investigated in sarcolemmal vesicles. Western blots of sarcolemmal vesicles revealed the presence of caveolin-1, -2, and -3. NCX1 co-fractionated more closely with caveolin-3 than caveolin-1 on sucrose density gradients. NCX1 has five possible caveolin-binding motifs and NCX1 co-precipitated specifically with caveolin-3. Molecular sieve column chromatography indicated that this co-precipitation was not due to incomplete solubilization of lipid raft microdomains. Cholesterol chelation in vesicles decreased NCX1 transport activity and caveolin-3 co-precipitation. NCX1 may play a role in caveolar transmembrane signaling in addition to its role in excitation-contraction coupling.

@article{Boss_2002_113, abstract = {The interaction of cardiac {N}a$^{+}$-{C}a$^{2+}$ exchange (NCX1) with caveolin proteins was investigated in sarcolemmal vesicles. Western blots of sarcolemmal vesicles revealed the presence of caveolin-1, -2, and -3. NCX1 co-fractionated more closely with caveolin-3 than caveolin-1 on sucrose density gradients. NCX1 has five possible caveolin-binding motifs and NCX1 co-precipitated specifically with caveolin-3. Molecular sieve column chromatography indicated that this co-precipitation was not due to incomplete solubilization of lipid raft microdomains. Cholesterol chelation in vesicles decreased NCX1 transport activity and caveolin-3 co-precipitation. NCX1 may play a role in caveolar transmembrane signaling in addition to its role in excitation-contraction coupling.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Bossuyt, Julie and Taylor, Bonnie E and James-Kracke, Marilyn and Hale, Calvin C}, biburl = {https://www.bibsonomy.org/bibtex/2614c6fac0a8e0ea11ac4a3f0360c56d7/hake}, description = {The whole bibliography file I use.}, doi = {10.1016/S0014-5793(01)03323-3}, file = {Boss_2002_113.pdf:Boss_2002_113.pdf:PDF}, institution = {The Dalton Cardiovascular Research Center, University of Missouri, Columbia, MO 65211, USA.}, interhash = {dd9137953203919bbde0abcf3671c3e4}, intrahash = {614c6fac0a8e0ea11ac4a3f0360c56d7}, journal = {FEBS Lett.}, keywords = {3; Acid Amino Animals; Binding Binding; Blotting, Cattle; Caveolae, Caveolin Caveolins, Centrifugation, Cholesterol, Chromatography, Cyclodextrins, Density Exchanger, Gel; Gradient; Motifs; Myocardium, Precipitin Protein Sarcolemma, Sites; Sodium-Calcium Tests; Western; chemistry/drug chemistry/metabolism effects/metabolism; metabolism; pharmacology;}, month = Jan, number = {1-3}, pages = {113--117}, pdf = {Boss_2002_113.pdf}, pii = {S0014579301033233}, pmid = {11821059}, timestamp = {2009-06-03T11:21:06.000+0200}, title = {Evidence for cardiac sodium-calcium exchanger association with caveolin-3.}, url = {http://dx.doi.org/10.1016/S0014-5793(01)03323-3}, volume = 511, year = 2002 }