%0 Journal Article %1 Soel_1999_266 %A Soeller, C. %A Cannell, M. B. %D 1999 %J Circ. Res. %K Acetic Acid Acid, Acids, Action Activation, Adaptation, Adenosine Adhesion, Adhesions, Agents, Algorithms, Allergens, Allergic, Alternative Amino Aniline Animals, Antibodies, Antibody Antigens, Antipain, Auditory Biological Biological, Biosensing Bronchi, Butyric Cadherins, Calcium Calcium, Cardiac, Cardiovascular, Carrier Cell Cells, Channels, Cheese, Chelating Cilia, Cochlea, Communication, Comparative Compartmentation, Compounds, Computer Computer-Assisted, Conductivity, Confocal, Connexins, Crystalline, Crystallins, Culture Cultured, Cysteine Dermatophagoides, Desmosomes, Diagnostic Differentiation, Diffusion, Dogs, Dyes, Electric Electrochemistry, Electrophysiology, Endopeptidases, Enzyme Epithelial Epithelium, Epitopes, Ethylenediamines, Feasibility Feces, Feedback, Female, Fluorescein, Fluorescein-5-isothiocyanate, Fluorescence, Fluorescent Focal Imaging, Impedance, Inhibitors, Line, Membrane Membrane, Perception, Permeability, Potentials, Proteins, Sequence, Signaling, Simulation, Splicing, Stimulation, Studies, Study, Technique, Techniques, Transport, Triphosphate, %N 3 %P 266-75 %T Examination of the transverse tubular system in living cardiac rat myocytes by 2-photon microscopy and digital image-processing techniques. %U http://circres.ahajournals.org/cgi/content/full/84/3/266 %V 84 %X The transverse tubular system (t-system) of cardiac muscle is a structure that allows rapid propagation of excitation into the cell interior. Using 2-photon molecular excitation microscopy and digital image-processing methods, we have obtained a comprehensive overview of the t-system of rat ventricular myocytes in living cells. We show that it is possible to quantify the morphology of the t-system in terms of average local tubule diameter, branching pattern, and local abundance of the t-system by immersing living myocytes in a dextran-linked fluorescein solution. Our data suggest that previous electron microscopic examinations of t-system structure have underestimated both the geometric complexity of the t-system morphology and the fraction of cell volume occupied by the t-system (3.6\% in this species). About 40\% of tubules occur between Z-lines, and the t-tubule diameter is 255+/-0.85 nm (mean+/-SEM). The t-tubules leave the outer surface of the cell in an approximately rectangular array; however, at some points junctions between the t-tubules and the surface membrane are missing. In view of the complexity of the t-system apparent from our images, we propose that the t-system be renamed the "sarcolemmal Z rete." The methods presented here are generally applicable to the quantification of the sarcolemmal Z rete and other structures within cells by fluorescence microscopy in a variety of cell types.

@article{Soel_1999_266, abstract = {The transverse tubular system (t-system) of cardiac muscle is a structure that allows rapid propagation of excitation into the cell interior. Using 2-photon molecular excitation microscopy and digital image-processing methods, we have obtained a comprehensive overview of the t-system of rat ventricular myocytes in living cells. We show that it is possible to quantify the morphology of the t-system in terms of average local tubule diameter, branching pattern, and local abundance of the t-system by immersing living myocytes in a dextran-linked fluorescein solution. Our data suggest that previous electron microscopic examinations of t-system structure have underestimated both the geometric complexity of the t-system morphology and the fraction of cell volume occupied by the t-system (3.6\% in this species). About 40\% of tubules occur between Z-lines, and the t-tubule diameter is 255+/-0.85 nm (mean+/-SEM). The t-tubules leave the outer surface of the cell in an approximately rectangular array; however, at some points junctions between the t-tubules and the surface membrane are missing. In view of the complexity of the t-system apparent from our images, we propose that the t-system be renamed the "sarcolemmal Z rete." The methods presented here are generally applicable to the quantification of the sarcolemmal Z rete and other structures within cells by fluorescence microscopy in a variety of cell types.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Soeller, C. and Cannell, M. B.}, biburl = {https://www.bibsonomy.org/bibtex/28e46d54d739814aed194a73478ef4c16/hake}, description = {The whole bibliography file I use.}, file = {Soel_1999_266.pdf:Soel_1999_266.pdf:PDF}, interhash = {ded921384b2879a03ad3a153913574af}, intrahash = {8e46d54d739814aed194a73478ef4c16}, journal = {Circ. Res.}, key = 50, keywords = {Acetic Acid Acid, Acids, Action Activation, Adaptation, Adenosine Adhesion, Adhesions, Agents, Algorithms, Allergens, Allergic, Alternative Amino Aniline Animals, Antibodies, Antibody Antigens, Antipain, Auditory Biological Biological, Biosensing Bronchi, Butyric Cadherins, Calcium Calcium, Cardiac, Cardiovascular, Carrier Cell Cells, Channels, Cheese, Chelating Cilia, Cochlea, Communication, Comparative Compartmentation, Compounds, Computer Computer-Assisted, Conductivity, Confocal, Connexins, Crystalline, Crystallins, Culture Cultured, Cysteine Dermatophagoides, Desmosomes, Diagnostic Differentiation, Diffusion, Dogs, Dyes, Electric Electrochemistry, Electrophysiology, Endopeptidases, Enzyme Epithelial Epithelium, Epitopes, Ethylenediamines, Feasibility Feces, Feedback, Female, Fluorescein, Fluorescein-5-isothiocyanate, Fluorescence, Fluorescent Focal Imaging, Impedance, Inhibitors, Line, Membrane Membrane, Perception, Permeability, Potentials, Proteins, Sequence, Signaling, Simulation, Splicing, Stimulation, Studies, Study, Technique, Techniques, Transport, Triphosphate,}, month = Feb, number = 3, pages = {266-75}, timestamp = {2009-06-03T11:21:31.000+0200}, title = {Examination of the transverse tubular system in living cardiac rat myocytes by 2-photon microscopy and digital image-processing techniques.}, url = {http://circres.ahajournals.org/cgi/content/full/84/3/266}, volume = 84, year = 1999 }