%0 Journal Article %1 lewis2005conserved %A Lewis, Benjamin P %A Burge, Christopher B %A Bartel, David P %D 2005 %J Cell %K 7 methods micrornas software %N 1 %P 15-20 %R 10.1016/j.cell.2004.12.035 %T Conserved Seed Pairing, Often Flanked by Adenosines, Indicates that Thousands of Human Genes are MicroRNA Targets %U http://www.sciencedirect.com/science/article/pii/S0092867404012607?via%3Dihub %V 120 %X We predict regulatory targets of vertebrate microRNAs (miRNAs) by identifying mRNAs with conserved complementarity to the seed (nucleotides 2-7) of the miRNA. An overrepresentation of conserved adenosines flanking the seed complementary sites in mRNAs indicates that primary sequence determinants can supplement base pairing to specify miRNA target recognition. In a four-genome analysis of 3' UTRs, approximately 13,000 regulatory relationships were detected above the estimate of false-positive predictions, thereby implicating as miRNA targets more than 5300 human genes, which represented 30% of our gene set. Targeting was also detected in open reading frames. In sum, well over one third of human genes appear to be conserved miRNA targets.

@article{lewis2005conserved, abstract = {We predict regulatory targets of vertebrate microRNAs (miRNAs) by identifying mRNAs with conserved complementarity to the seed (nucleotides 2-7) of the miRNA. An overrepresentation of conserved adenosines flanking the seed complementary sites in mRNAs indicates that primary sequence determinants can supplement base pairing to specify miRNA target recognition. In a four-genome analysis of 3' UTRs, approximately 13,000 regulatory relationships were detected above the estimate of false-positive predictions, thereby implicating as miRNA targets more than 5300 human genes, which represented 30% of our gene set. Targeting was also detected in open reading frames. In sum, well over one third of human genes appear to be conserved miRNA targets.}, added-at = {2017-10-19T19:59:24.000+0200}, author = {Lewis, Benjamin P and Burge, Christopher B and Bartel, David P}, biburl = {https://www.bibsonomy.org/bibtex/2aaa99c25d39f080f039afeeb6f16ec63/artheibault}, description = {This article describes the conserved seed method used by TargetScan. This method is used to determine microRNA binding sites in a genetic sequence.}, doi = {10.1016/j.cell.2004.12.035}, interhash = {e1aa12a5affa71bd272721f76c66bf46}, intrahash = {aaa99c25d39f080f039afeeb6f16ec63}, journal = {Cell}, keywords = {7 methods micrornas software}, month = jan, number = 1, pages = {15-20}, timestamp = {2017-10-25T21:37:25.000+0200}, title = {Conserved Seed Pairing, Often Flanked by Adenosines, Indicates that Thousands of Human Genes are MicroRNA Targets}, url = {http://www.sciencedirect.com/science/article/pii/S0092867404012607?via%3Dihub}, volume = 120, year = 2005 }