%0 Journal Article %1 citeulike:469296 %A Paul, A. %A Madan, S. %A Vasandani, V. M. %A Ghosh, P. C. %A Bachhawat, B. K. %C Department of Biochemistry, University of Delhi, India. %D 1992 %J J Immunol Methods %K l-i-l-a complement immunoassay homogeneous ag_detection %N 1-2 %P 151--158 %T Liposome immune lysis assay (LILA) for gelonin. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1564325 %V 148 %X A complement-mediated liposome immune lysis assay using entrapped calcein was developed for a plant toxin gelonin. Gelonin was covalently coupled to DPPE, and then adsorbed on to the surface of liposomes. Such antigen-bearing liposomes when incubated with anti-gelonin antibody in the presence of guinea pig complement undergo lysis. The detection range is from 3 ng to 60 ng. The method was used to monitor isolation of gelonin by affinity chromatography. It was observed that a minor peak in addition to the major one comes with gelonin, shared common epitopes/epitope with gelonin in immunological reaction. This was further confirmed by SDS-gel electrophoresis indicating the former being an isoform of gelonin. A comparative study of the immunocross-reactivity of ricin and ricin A chain with anti-gelonin antibody was carried out. It was found that while ricin A chain cross-reacted extensively with gelonin antibody and intact ricin elicited little or no cross-reactivity. It is suggested that the present LILA may be employed for the detection and quantitation of ricin A chain by this LILA method.

@article{citeulike:469296, abstract = {A complement-mediated liposome immune lysis assay using entrapped calcein was developed for a plant toxin gelonin. Gelonin was covalently coupled to DPPE, and then adsorbed on to the surface of liposomes. Such antigen-bearing liposomes when incubated with anti-gelonin antibody in the presence of guinea pig complement undergo lysis. The detection range is from 3 ng to 60 ng. The method was used to monitor isolation of gelonin by affinity chromatography. It was observed that a minor peak in addition to the major one comes with gelonin, shared common epitopes/epitope with gelonin in immunological reaction. This was further confirmed by SDS-gel electrophoresis indicating the former being an isoform of gelonin. A comparative study of the immunocross-reactivity of ricin and ricin A chain with anti-gelonin antibody was carried out. It was found that while ricin A chain cross-reacted extensively with gelonin antibody and intact ricin elicited little or no cross-reactivity. It is suggested that the present LILA may be employed for the detection and quantitation of ricin A chain by this LILA method.}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Department of Biochemistry, University of Delhi, India.}, author = {Paul, A. and Madan, S. and Vasandani, V. M. and Ghosh, P. C. and Bachhawat, B. K.}, biburl = {https://www.bibsonomy.org/bibtex/2884673aef0542c7a25873a64d6372567/biblio24}, citeulike-article-id = {469296}, interhash = {e3f638ed7c396f88ee9e5b8e526984e6}, intrahash = {884673aef0542c7a25873a64d6372567}, issn = {0022-1759}, journal = {J Immunol Methods}, keywords = {l-i-l-a complement immunoassay homogeneous ag_detection}, month = {April}, number = {1-2}, pages = {151--158}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Liposome immune lysis assay (LILA) for gelonin.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=1564325}, volume = 148, year = 1992 }