Abstract
\textlessp\textgreater\textlessbr/\textgreaterReconstructing
neuronal circuits at the level of synapses is a central
problem in neuroscience, and the focus of the nascent field of
connectomics. Previously used to reconstruct the C. elegans
wiring diagram, serial-section transmission electron
microscopy (ssTEM) is a proven technique for the
task. However, to reconstruct more complex circuits, ssTEM
will require the automation of image processing. We review
progress in the processing of electron microscopy images and,
in particular, a semi-automated reconstruction pipeline
deployed at Janelia Farm. Drosophila circuits underlying
identified behaviors are being reconstructed in the pipeline
with the goal of generating a complete Drosophila
connectome.\textless/p\textgreater
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