%0 Journal Article %1 Ries2012 %A Ries, Jonas %A Kaplan, Charlotte %A Platonova, Evgenia %A Eghlidi, Hadi %A Ewers, Helge %D 2012 %J Nature Methods %K antibodies fluorescence fluorophores gfp microscopy nanobodies superresolution %N 6 %P 582--584 %R 10.1038/nmeth.1991 %T A simple, versatile method for GFP-based super-resolution microscopy via nanobodies %U http://www.nature.com/doifinder/10.1038/nmeth.1991. %V 9 %X We developed a method to use any GFP-tagged construct in single-molecule super-resolution microscopy. By targeting GFP with small, high-affinity antibodies coupled to organic dyes, we achieved nanometer spatial resolution and minimal linkage error when analyzing microtubules, living neurons and yeast cells. We show that in combination with libraries encoding GFP-tagged proteins, virtually any known protein can immediately be used in super-resolution microscopy and that simplified labeling schemes allow high-throughput super-resolution imaging. © 2012 Nature America, Inc. All rights reserved.

@article{Ries2012, abstract = {We developed a method to use any GFP-tagged construct in single-molecule super-resolution microscopy. By targeting GFP with small, high-affinity antibodies coupled to organic dyes, we achieved nanometer spatial resolution and minimal linkage error when analyzing microtubules, living neurons and yeast cells. We show that in combination with libraries encoding GFP-tagged proteins, virtually any known protein can immediately be used in super-resolution microscopy and that simplified labeling schemes allow high-throughput super-resolution imaging. {\textcopyright} 2012 Nature America, Inc. All rights reserved.}, added-at = {2020-03-23T21:12:34.000+0100}, author = {Ries, Jonas and Kaplan, Charlotte and Platonova, Evgenia and Eghlidi, Hadi and Ewers, Helge}, biburl = {https://www.bibsonomy.org/bibtex/20dfe5f685545b218d4d5d21b33c15a9f/kfriedl}, doi = {10.1038/nmeth.1991}, file = {:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Ries et al. - 2012 - a simple, versatile method for GfP-based super-resolution microscopy via nanobodies.pdf:pdf}, interhash = {e51a34e88eb6e9136a6c7d68641c7298}, intrahash = {0dfe5f685545b218d4d5d21b33c15a9f}, issn = {15487091}, journal = {Nature Methods}, keywords = {antibodies fluorescence fluorophores gfp microscopy nanobodies superresolution}, number = 6, pages = {582--584}, pmid = {22543348}, timestamp = {2020-04-07T11:44:26.000+0200}, title = {{A simple, versatile method for GFP-based super-resolution microscopy via nanobodies}}, url = {http://www.nature.com/doifinder/10.1038/nmeth.1991.}, volume = 9, year = 2012 }