%0 Journal Article %1 perron_sustained_2006 %A Perron, Amélie %A Sharif, Nadder %A Gendron, Louis %A Lavallée, Mariette %A Stroh, Thomas %A Mazella, Jean %A Beaudet, Alain %D 2006 %J Biochemical and Biophysical Research Communications %K Animals Cell_Membrane Cercopithecus_aethiops Golgi_Apparatus Humans Kinetics Male Neurons Neurotensin Protein_Transport Rats Receptors Spinal_Cord {COS}_Cells {Down-Regulation} {Sprague-Dawley} %N 3 %P 799--808 %R 10.1016/j.bbrc.2006.03.047 %T Sustained neurotensin exposure promotes cell surface recruitment of NTS2 receptors %U http://www.ncbi.nlm.nih.gov/pubmed/16564027 %V 343 %X In this study, we investigated whether persistent agonist stimulation of NTS2 receptors gives rise to down-regulation, in light of reports that their activation induced long-lasting effects. To address this issue, we incubated COS-7 cells expressing the rat NTS2 with neurotensin (NT) for up to 24 h and measured resultant cell surface 125I-NT binding. We found that NTS2-expressing cells retained the same surface receptor density despite efficient internalization mechanisms. This preservation was neither due to NTS2 neosynthesis nor recycling since it was not blocked by cycloheximide or monensin. However, it appeared to involve translocation of spare receptors from internal stores, as NT induced NTS2 migration from trans-Golgi network to endosome-like structures. This stimulation-induced regulation of cell surface NTS2 receptors was even more striking in rat spinal cord neurons. Taken together, these results suggest that sustained NTS2 activation promotes recruitment of intracellular receptors to the cell surface, thereby preventing functional desensitization.

@article{perron_sustained_2006, abstract = {In this study, we investigated whether persistent agonist stimulation of {NTS2} receptors gives rise to down-regulation, in light of reports that their activation induced long-lasting effects. To address this issue, we incubated {COS-7} cells expressing the rat {NTS2} with neurotensin {(NT)} for up to 24 h and measured resultant cell surface {[125I]-NT} binding. We found that {NTS2-expressing} cells retained the same surface receptor density despite efficient internalization mechanisms. This preservation was neither due to {NTS2} neosynthesis nor recycling since it was not blocked by cycloheximide or monensin. However, it appeared to involve translocation of spare receptors from internal stores, as {NT} induced {NTS2} migration from {trans-Golgi} network to endosome-like structures. This stimulation-induced regulation of cell surface {NTS2} receptors was even more striking in rat spinal cord neurons. Taken together, these results suggest that sustained {NTS2} activation promotes recruitment of intracellular receptors to the cell surface, thereby preventing functional desensitization.}, added-at = {2011-08-03T21:06:35.000+0200}, author = {Perron, Amélie and Sharif, Nadder and Gendron, Louis and Lavallée, Mariette and Stroh, Thomas and Mazella, Jean and Beaudet, Alain}, biburl = {https://www.bibsonomy.org/bibtex/21845c4a63a87e015830afa2c6998762d/crc_chus}, doi = {10.1016/j.bbrc.2006.03.047}, interhash = {eec986a032a6eb175efc3290fa1e948d}, intrahash = {1845c4a63a87e015830afa2c6998762d}, issn = {{0006-291X}}, journal = {Biochemical and Biophysical Research Communications}, keywords = {Animals Cell_Membrane Cercopithecus_aethiops Golgi_Apparatus Humans Kinetics Male Neurons Neurotensin Protein_Transport Rats Receptors Spinal_Cord {COS}_Cells {Down-Regulation} {Sprague-Dawley}}, month = may, note = {{PMID:} 16564027}, number = 3, pages = {799--808}, timestamp = {2011-08-03T21:10:04.000+0200}, title = {Sustained neurotensin exposure promotes cell surface recruitment of {NTS2} receptors}, url = {http://www.ncbi.nlm.nih.gov/pubmed/16564027}, volume = 343, year = 2006 }