%0 Journal Article %1 Kawa_2000_189 %A Kawano, S. %A Hiraoka, M. %D 2000 %J Receptors Channels %K ATPase, Acidosis, Action Adipocytes, Adolescent, Adrenergic Adult, Agents, Animals, Anti-Arrhythmia Arrhythmia, Atrial, Base Biological Biological, Block, Blockers, Body Bone Bundle-Branch COS Calcium Calcium, Calcium-Activated, Calmodulin, Cardiac, Cardiomyopathies, Cation Cell Cells, Channel Channels, Child, Chloride Comparative Concentration, Conduction Conductivity, Confocal, Cultured, Cytoplasmic DNA DNA-Binding Defects, Differentiation, Dominant, Electric Electrocardiography, Electromagnetic Electrophysiology, Endocardium, Endoplasmic Enhancement, Female, Fields, Frameshift G-Protein-Coupled, Gating, Genes, Hamsters, Heart Heart, Hematopoietic Humans, Hydrogen-Ion Image Imidazoles, Ion Isoproterenol, Kinetics, L-Type, Lidocaine, Line, Lipase, Lipoprotein Long Mapping, Markers, Marrow Membrane, Mutation, Nuclear, Nucleus, Potential Potentials, Primers, Proteins, Reticulum, Septal Sequence, Signaling, Size, Stem Study, Surface System, Transport Ventricles, and beta-Agonists, {C}a$^{2+}$-Transporting %N 3 %P 189--197 %T Voltage-dependent effects of Ca$^2+$/calmodulin on Cl$^-$ channel in cardiac sarcoplasmic reticulum. %V 7 %X A new kind of chloride channels in the cardiac sarcoplasmic reticulum, 116 pS Cl$^-$ channel (500 mM Cl$^-$ in the cis and 50 mM Cl$^-$ in the trans chamber solutions), which is activated by protein-kinase-A-dependent phosphorylation, has been determined to conduct adenine nucleotide as a transporter between cytosol and SR lumen. We investigated the voltage-dependent gating of this Cl$^-$ channel by recording single-channel activities using the planar lipid bilayer-vesicle fusion technique. The channel activities did not change at different membrane potentials (-100 mV to +50 mV) or different Ca$^2+$ concentrations (1 nM to 1 mM) in cis solution. In the presence of calmodulin (CaM) (0.1 microM /microg SR vesicles), however, Ca$^2+$ added to the cis solution at 0 mV inhibited channel openings in a Ca$^2+$ -concentration-dependent manner. These effects were prevented by the addition of CaM inhibitors. The blocking effects of CaM differed depending on the membrane potentials at negative potentials below -20 mV. With CaM and 3 microM Ca$^2+$, the values of opening probability were 0 at -80 mV, 0.2 at -40 mV, 0.3 at -20 mV, 0.71 at 0 mV and 0.92 at +20 mV. These results may indicate the membrane potential affects the action of Ca$^2+$ /CaM complex

@article{Kawa_2000_189, abstract = {A new kind of chloride channels in the cardiac sarcoplasmic reticulum, 116 pS {C}l$^{-}$ channel (500 mM {C}l$^{-}$ in the cis and 50 mM {C}l$^{-}$ in the trans chamber solutions), which is activated by protein-kinase-A-dependent phosphorylation, has been determined to conduct adenine nucleotide as a transporter between cytosol and SR lumen. We investigated the voltage-dependent gating of this {C}l$^{-}$ channel by recording single-channel activities using the planar lipid bilayer-vesicle fusion technique. The channel activities did not change at different membrane potentials (-100 mV to +50 mV) or different {C}a$^{2+}$ concentrations (1 nM to 1 mM) in cis solution. In the presence of calmodulin (CaM) (0.1 microM /microg SR vesicles), however, {C}a$^{2+}$ added to the cis solution at 0 mV inhibited channel openings in a {C}a$^{2+}$ -concentration-dependent manner. These effects were prevented by the addition of CaM inhibitors. The blocking effects of CaM differed depending on the membrane potentials at negative potentials below -20 mV. With CaM and 3 microM {C}a$^{2+}$, the values of opening probability were 0 at -80 mV, 0.2 at -40 mV, 0.3 at -20 mV, 0.71 at 0 mV and 0.92 at +20 mV. These results may indicate the membrane potential affects the action of {C}a$^{2+}$ /CaM complex}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Kawano, S. and Hiraoka, M.}, biburl = {https://www.bibsonomy.org/bibtex/2f9211dc5fc7fcf3aaa9f85f6cbb3f670/hake}, description = {The whole bibliography file I use.}, interhash = {f24b584a6168528a686aae2c24631791}, intrahash = {f9211dc5fc7fcf3aaa9f85f6cbb3f670}, journal = {Receptors Channels}, key = 204, keywords = {ATPase, Acidosis, Action Adipocytes, Adolescent, Adrenergic Adult, Agents, Animals, Anti-Arrhythmia Arrhythmia, Atrial, Base Biological Biological, Block, Blockers, Body Bone Bundle-Branch COS Calcium Calcium, Calcium-Activated, Calmodulin, Cardiac, Cardiomyopathies, Cation Cell Cells, Channel Channels, Child, Chloride Comparative Concentration, Conduction Conductivity, Confocal, Cultured, Cytoplasmic DNA DNA-Binding Defects, Differentiation, Dominant, Electric Electrocardiography, Electromagnetic Electrophysiology, Endocardium, Endoplasmic Enhancement, Female, Fields, Frameshift G-Protein-Coupled, Gating, Genes, Hamsters, Heart Heart, Hematopoietic Humans, Hydrogen-Ion Image Imidazoles, Ion Isoproterenol, Kinetics, L-Type, Lidocaine, Line, Lipase, Lipoprotein Long Mapping, Markers, Marrow Membrane, Mutation, Nuclear, Nucleus, Potential Potentials, Primers, Proteins, Reticulum, Septal Sequence, Signaling, Size, Stem Study, Surface System, Transport Ventricles, and beta-Agonists, {C}a$^{2+}$-Transporting}, number = 3, pages = {189--197}, pii = {I392J001004}, pmid = {11342387}, timestamp = {2009-06-03T11:21:17.000+0200}, title = {Voltage-dependent effects of {C}a$^{2+}$/calmodulin on {C}l$^-$ channel in cardiac sarcoplasmic reticulum.}, volume = 7, year = 2000 }