Abstract
Sensors based on fluorescence resonance energy transfer (FRET) are
powerful tools to monitor signaling events in living mammalian cells.
Here we describe development and use of new sensors for cyclic GMP
(cGMP) based on cGMP binding domains from cGMP-dependent protein
kinase I (GKI) and from phosphodiesterases (PDEs). The temporal and
spatial resolution attained with the new sensors is superior to that
of existing techniques, and permits direct recording and imaging
of rapid cGMP-signaling events.
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