%0 Journal Article %1 Bere_2007_1452 %A Berecki, G�za %A Ruijter, Hester M Den %A Verkerk, Arie O %A Schumacher, Cees A %A Baartscheer, Antonius %A Bakker, Diane %A Boukens, Bastiaan J %A van Ginneken, Antoni C G %A Fiolet, Jan W T %A Opthof, Tobias %A Coronel, Ruben %D 2007 %J Heart Rhythm %K /&/ Action Animals; Arrhythmias, Calcium Cardiac, Cells, Channels, Death, Dust; Factors Factors; Fish Heart Incidence; Male; Membrane Muscle Nutritional Oils, Phospholipids; Potentials, Potentials; Risk Status; Sudden, Swine; Time Ventricles, control; drug effects; pharmacology; prevention %N 11 %P 1452--1460 %R 10.1016/j.hrthm.2007.07.015 %T Dietary fish oil reduces the incidence of triggered arrhythmias in pig ventricular myocytes. %U http://dx.doi.org/10.1016/j.hrthm.2007.07.015 %V 4 %X BACKGROUND: Fish oil reduces the incidence of sudden cardiac death in postmyocardial infarction patients. Triggered activity is the principal mechanism of arrhythmogenesis under these conditions. OBJECTIVE: The purpose of this study was to test whether dietary fish oil in pigs inhibits Ca2+ overload-induced triggered activity. METHODS: Pigs were fed a diet of fish oil or sunflower oil for 8 weeks. Ventricular myocytes (omega3: fish oil, n = 11; control: sunflower oil, n = 8) were isolated by enzymatic dissociation and used for patch clamp studies and intracellular Ca2+ recordings. Triggered activity was induced by rapid pacing in the presence of norepinephrine. RESULTS: Dietary fish oil reduced the incidence of triggered action potentials and delayed afterdepolarizations compared to control (9.1\% in omega3 and 84.6\% in control, P <.05), concomitant with a reduction in spontaneous Ca2+ release. Dietary fish oil prevented Ca2+ overload and reduced action potential prolongation in response to norepinephrine (DeltaAPD(90): 23.2 +/- 8.5 ms in omega3 and 107.4 +/- 15.9 in control, P <.05). omega3 myocytes displayed decreased sarcoplasmic reticulum Ca2+ content, reduced L-type Ca2+ current (I(Ca,L)), and less recruitment of the Na+/Ca2+ exchange current (I(NCX)) in response to norepinephrine compared to control. In the absence of norepinephrine, the slow component of the delayed rectifier current (I(Ks)) was larger in omega3 myocytes. In the presence of norepinephrine, I(Ks) increased to the same level in omega3 and control myocytes. CONCLUSION: Dietary fish oil reduces the incidence of triggered activity and prevents Ca2+ overload and AP prolongation in response to norepinephrine. Fish oil may prevent arrhythmias in patients with heart failure.

@article{Bere_2007_1452, abstract = {BACKGROUND: Fish oil reduces the incidence of sudden cardiac death in postmyocardial infarction patients. Triggered activity is the principal mechanism of arrhythmogenesis under these conditions. OBJECTIVE: The purpose of this study was to test whether dietary fish oil in pigs inhibits Ca2+ overload-induced triggered activity. METHODS: Pigs were fed a diet of fish oil or sunflower oil for 8 weeks. Ventricular myocytes (omega3: fish oil, n = 11; control: sunflower oil, n = 8) were isolated by enzymatic dissociation and used for patch clamp studies and intracellular Ca2+ recordings. Triggered activity was induced by rapid pacing in the presence of norepinephrine. RESULTS: Dietary fish oil reduced the incidence of triggered action potentials and delayed afterdepolarizations compared to control (9.1\% in omega3 and 84.6\% in control, P <.05), concomitant with a reduction in spontaneous Ca2+ release. Dietary fish oil prevented Ca2+ overload and reduced action potential prolongation in response to norepinephrine (DeltaAPD(90): 23.2 +/- 8.5 ms in omega3 and 107.4 +/- 15.9 in control, P <.05). omega3 myocytes displayed decreased sarcoplasmic reticulum Ca2+ content, reduced L-type Ca2+ current (I(Ca,L)), and less recruitment of the Na+/Ca2+ exchange current (I(NCX)) in response to norepinephrine compared to control. In the absence of norepinephrine, the slow component of the delayed rectifier current (I(Ks)) was larger in omega3 myocytes. In the presence of norepinephrine, I(Ks) increased to the same level in omega3 and control myocytes. CONCLUSION: Dietary fish oil reduces the incidence of triggered activity and prevents Ca2+ overload and AP prolongation in response to norepinephrine. Fish oil may prevent arrhythmias in patients with heart failure.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Berecki, G�za and Ruijter, Hester M Den and Verkerk, Arie O and Schumacher, Cees A and Baartscheer, Antonius and Bakker, Diane and Boukens, Bastiaan J and van Ginneken, Antoni C G and Fiolet, Jan W T and Opthof, Tobias and Coronel, Ruben}, biburl = {https://www.bibsonomy.org/bibtex/2989258709999684cb2cc3473949dabfb/hake}, description = {The whole bibliography file I use.}, doi = {10.1016/j.hrthm.2007.07.015}, file = {Bere_2007_1452.pdf:Bere_2007_1452.pdf:PDF}, institution = {Experimental Cardiology Group (ECG), Center for Heart Failure Research, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.}, interhash = {fb21b3c930cca15c54a7838a98249d24}, intrahash = {989258709999684cb2cc3473949dabfb}, journal = {Heart Rhythm}, keywords = {/&/ Action Animals; Arrhythmias, Calcium Cardiac, Cells, Channels, Death, Dust; Factors Factors; Fish Heart Incidence; Male; Membrane Muscle Nutritional Oils, Phospholipids; Potentials, Potentials; Risk Status; Sudden, Swine; Time Ventricles, control; drug effects; pharmacology; prevention}, month = Nov, number = 11, pages = {1452--1460}, pdf = {Bere_2007_1452.pdf}, pii = {S1547-5271(07)00727-8}, pmid = {17954406}, timestamp = {2009-06-03T11:21:02.000+0200}, title = {Dietary fish oil reduces the incidence of triggered arrhythmias in pig ventricular myocytes.}, url = {http://dx.doi.org/10.1016/j.hrthm.2007.07.015}, volume = 4, year = 2007 }