%0 Journal Article %1 Ohta1988 %A Ohta, Takafumi %A Goto, Nozomi %A Takitani, Shoji %D 1988 %J Analyst %K absorbance flow-injection hardware nitroso %P 1333 %R 10.1039/AN9881301333 %T Spectrophotometric Determination of n-Nitroso Compounds by Flow Injection Analysis %V 113 %X A spectrophotometric method for the determination of N-nitroso compounds by flow injection analysis, based on a denitrosation reaction with hydrogen bromide - acetic acid followed by reaction of the liberated nitrite with the Griess reagent, is described. Under the optimum reaction conditions, the detection limit for N-nitrosopyrrolidine (NPYR) was 5 x 10^-8 M for a sample volume of 200 pl and the relative standard deviations (n = 9) for 1 x 10^-7 and 5.4 x 10^-6 M NPYR were 3.8 and 0.5%, respectively. Various other N-nitroso compounds gave a similar level of response. Phenol, sorbic acid and methanol did not interfere with the determinations, whereas piperazine interfered seriously with the determination of nitrosoproline. The sampling rate was 25 samples h-1. The method was successfully applied to the analysis of two anti-cancer drug formulations, viz., nimustine hydrochloride and l-(2-chloroethyl)-3-(methyl-alpha-D-glucopyranos-6-yl)-1-nitrosourea injections.

@article{Ohta1988, abstract = {A spectrophotometric method for the determination of N-nitroso compounds by flow injection analysis, based on a denitrosation reaction with hydrogen bromide - acetic acid followed by reaction of the liberated nitrite with the Griess reagent, is described. Under the optimum reaction conditions, the detection limit for N-nitrosopyrrolidine (NPYR) was 5 x 10^-8 M for a sample volume of 200 pl and the relative standard deviations (n = 9) for 1 x 10^-7 and 5.4 x 10^-6 M NPYR were 3.8 and 0.5%, respectively. Various other N-nitroso compounds gave a similar level of response. Phenol, sorbic acid and methanol did not interfere with the determinations, whereas piperazine interfered seriously with the determination of nitrosoproline. The sampling rate was 25 samples h-1. The method was successfully applied to the analysis of two anti-cancer drug formulations, viz., nimustine hydrochloride and l-(2-chloroethyl)-3-(methyl-alpha-D-glucopyranos-6-yl)-1-nitrosourea injections.}, added-at = {2010-03-09T21:44:07.000+0100}, author = {Ohta, Takafumi and Goto, Nozomi and Takitani, Shoji}, biburl = {https://www.bibsonomy.org/bibtex/2bebeb1d86990d272ef0dabba370f95d3/afcallender}, doi = {10.1039/AN9881301333}, file = {Ohta1988.pdf:indexed\\Ohta1988.pdf:PDF}, interhash = {fd6c765a6084e53229f3f9c854c87365}, intrahash = {bebeb1d86990d272ef0dabba370f95d3}, journal = {Analyst}, keywords = {absorbance flow-injection hardware nitroso}, note = {teaching}, pages = 1333, timestamp = {2010-03-09T22:21:42.000+0100}, title = {Spectrophotometric Determination of n-Nitroso Compounds by Flow Injection Analysis}, volume = 113, year = 1988 }