%0 Journal Article %1 Dronov2007 %A Dronov, Roman %A Kurth, Dirk G. %A Moehwald, Helmuth %A Scheller, Frieder W. %A Lisdat, Fred %C THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND %D 2007 %I PERGAMON-ELSEVIER SCIENCE LTD %J ELECTROCHIMICA ACTA %K dgkurth %N 3 %P 1107-1113 %R 10.1016/j.electacta.2007.02.044 %T A self-assembled cytochrome c/xanthine oxidase multilayer arrangement on gold %U http://dx.doi.org/10.1016/j.electacta.2007.02.044 %V 53 %X A polyelectrolyte multilayer combining cytochrome c (cyt.c) and xanthine oxidase (XOD) is assembled on a gold electrode and investigated with respect to a signal chain formation from a xanthine oxidase substrate in solution. The multilayer assembly is prepared by means of an electrostatic self-assembly technique and consists of two parts each comprising one type of protein, which is responsible for a specific function. The outer part of the film contains XOD immobilized within poly(ethylenimin) layers and is responsible for selectivity towards hypoxanthine (HX) by its enzymatic conversion to uric acid. The inner layers contain cyt.c molecules embedded into a sulfonated polyaniline (PASA) matrix. The signal transfer mechanism within the assembly is proposed to be a mediated one. Thus, cyt.c plays a role of an internal transducer translating a HX concentration into an amperometric electrode response. Formation of the multilayer structure is confirmed by surface plasmon resonance (SPR), electrochemical experiments and UV-vis spectrophotometry. Influence of the multilayer composition on sensor performance is discussed. (c) 2007 Elsevier Ltd. All rights reserved.

@article{Dronov2007, abstract = {A polyelectrolyte multilayer combining cytochrome c (cyt.c) and xanthine oxidase (XOD) is assembled on a gold electrode and investigated with respect to a signal chain formation from a xanthine oxidase substrate in solution. The multilayer assembly is prepared by means of an electrostatic self-assembly technique and consists of two parts each comprising one type of protein, which is responsible for a specific function. The outer part of the film contains XOD immobilized within poly(ethylenimin) layers and is responsible for selectivity towards hypoxanthine (HX) by its enzymatic conversion to uric acid. The inner layers contain cyt.c molecules embedded into a sulfonated polyaniline (PASA) matrix. The signal transfer mechanism within the assembly is proposed to be a mediated one. Thus, cyt.c plays a role of an internal transducer translating a HX concentration into an amperometric electrode response. Formation of the multilayer structure is confirmed by surface plasmon resonance (SPR), electrochemical experiments and UV-vis spectrophotometry. Influence of the multilayer composition on sensor performance is discussed. (c) 2007 Elsevier Ltd. All rights reserved.}, added-at = {2011-01-12T12:39:21.000+0100}, address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND}, affiliation = {Lisdat, F (Reprint Author), Max Planck Inst Colloids \& Interfaces, Am Muhlenberg 1, D-14424 Potsdam, Germany. Max Planck Inst Colloids \& Interfaces, D-14424 Potsdam, Germany. Univ Potsdam, Inst Biochem \& Biol, D-14476 Golm, Germany. Wildau Univ Appl Sci Biosyst Technol, D-15745 Wildau, Germany.}, author = {Dronov, Roman and Kurth, Dirk G. and Moehwald, Helmuth and Scheller, Frieder W. and Lisdat, Fred}, author-email = {flisdat@igw.tfh-wildau.de}, biburl = {https://www.bibsonomy.org/bibtex/267d1fee4d5b6ff44fa0aab14c666a00f/lctm}, cited-references = {ANTIPOV AA, 2004, ADV COLLOID INTERFAC, V111, P49, DOI 10.1016/j.cis.2004.07.006. BEISSENHIRTZ MK, 2004, ANAL CHEM, V76, P4665, DOI 10.1021/ac049738f. BEISSENHIRTZ MK, 2004, ANGEW CHEM INT EDIT, V43, P4357, DOI 10.1002/anie.200352804. BEISSENHIRTZ MK, 2005, ELECTROANAL, V17, P1931, DOI 10.1002/elan.200503319. CALVO EJ, 2000, FARADAY DISCUSS, P67. CALVO EJ, 2000, FARADAY DISCUSS, V116, P47. CALVO EJ, 2002, J AM CHEM SOC, V124, P2452. CALVO EJ, 2004, CHEMPHYSCHEM, V5, P235, DOI 10.1002/cphc.200300930. CARUSO F, 1997, LANGMUIR, V13, P3422. CHANIOTAKIS NA, 2004, ANAL BIOANAL CHEM, V378, P89, DOI 10.1007/s00216-003-2188-3. CHAUBEY A, 2000, ANAL CHIM ACTA, V407, P97. DAWYDOFF W, 1991, ACTA POLYM, V42, P592. DECHER G, 1992, THIN SOLID FILMS, V210, P831. HEPTINSTALL S, 2005, J THROMB HAEMOST, V3, P2331. IGNATOV S, 2002, BIOSENS BIOELECTRON, V17, P191. JESSEL N, 2003, J ADV MAT, V15, P692. JIN YD, 2003, LANGMUIR, V19, P4771, DOI 10.1021/la026873j. KOTZ J, 1986, ACTA POLYM, V37, P108. KRONING S, 2004, ELECTROANAL, V16, P253, DOI 10.1002/elan.200402780. KRYLOV AV, 2004, ANAL BIOANAL CHEM, V378, P1327, DOI 10.1007/s00216-003-2426-8. LIU SQ, 2004, ANAL CHEM, V76, P4579, DOI 10.1021/ac0495283. LU SF, 2003, ANAL SCI, V19, P1309. LVOV Y, 1994, LANGMUIR, V10, P4232. LVOV Y, 1998, J BIOMAT SCI-POLYM E, V9, P345. MAO LQ, 2001, ANAL BIOCHEM, V292, P94. MELLO LD, 2002, FOOD CHEM, V77, P237. MULCHANDANI A, 1989, ANAL CHIM ACTA, V221, P215. NAKATANI HS, 2005, AM J BIOCH BIOTECHNO, V1, P85. PARK IS, 2000, ANAL CHIM ACTA, V404, P75. PEI JH, 2000, ANAL CHIM ACTA, V414, P205. RAM MK, 2001, BIOSENS BIOELECTRON, V16, P849. SCHELLER W, 1999, ELECTROANAL, V11, P703. STEITZ R, 2002, APPL PHYS A-MATE S 1, V74, S519, DOI 10.1007/s003390201782. TAKADA M, 2005, J CLIN PHARM THER, V30, P407. TARR HLA, 1966, J FOOD SCI, V31, P846. VENUGOPAL V, 2002, BIOSENS BIOELECTRON, V17, P147. WATANABE E, 2005, BIOSENS BIOELECTRON, V21, P534, DOI 10.1016/j.bios.2004.11.021. XUE HG, 2002, TALANTA, V57, P289. YAO T, 1993, ANAL CHIM ACTA, V281, P323.}, doc-delivery-number = {236LU}, doi = {10.1016/j.electacta.2007.02.044}, interhash = {fe00e3f0b60f55df8750f329165ca069}, intrahash = {67d1fee4d5b6ff44fa0aab14c666a00f}, issn = {0013-4686}, journal = {ELECTROCHIMICA ACTA}, journal-iso = {Electrochim. Acta}, keywords = {dgkurth}, keywords-plus = {CATIONIC POLYELECTROLYTES; SUPEROXIDE SENSOR; XANTHINE-OXIDASE; ELECTRODE; FILMS; HYPOXANTHINE; BIOSENSOR; FISH; SURFACES; DEPOSITION}, language = {English}, month = {DEC 20}, number = 3, number-of-cited-references = {39}, pages = {1107-1113}, publisher = {PERGAMON-ELSEVIER SCIENCE LTD}, subject-category = {Electrochemistry}, times-cited = {16}, timestamp = {2011-01-12T12:39:21.000+0100}, title = {A self-assembled cytochrome c/xanthine oxidase multilayer arrangement on gold}, type = {Proceedings Paper}, unique-id = {ISI:000251305300009}, url = {http://dx.doi.org/10.1016/j.electacta.2007.02.044}, volume = 53, year = 2007 }