The present experiment was conducted to determine the ideal concentration of different plant growth regulators (BA, Kin, IBA, IAA, and NAA) for in vitro regeneration of Caladium bicolor using shoot tip explants. The work was designed in CRD with three replications. Shoot tip explants gave rise to multiple shoots when cultured on MS medium supplemented with different concentration of BA with IBA. The highest (90%) response of shoot multiplication was obtained in MS medium containing 0.25-1.0mg/L BA + 2.0-2.5mg/L IBA.