%0 Journal Article %1 valenza_first_2010-1 %A Valenza, Giuseppe %A Joseph, Biju %A Elias, Johannes %A Claus, Heike %A Oesterlein, Anett %A Engelhardt, Kathrin %A Turnwald, Doris %A Frosch, Matthias %A Abele-Horn, Marianne %A Schoen, Christoph %D 2010 %J Antimicrob. Agents Chemother. %K imported %N 8 %P 3493--3497 %R 10.1128/AAC.00080-10 %T First Survey of Metallo-beta-Lactamases in Clinical Isolates of Pseudomonas aeruginosa in a German University Hospital %U http://aac.asm.org/cgi/content/abstract/54/8/3493 %V 54 %X A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-beta-lactamase (MBL) production. Molecular analysis detected a blaVIM-1 gene in the chromosome of one isolate and a blaVIM-2 gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory EDTA combination disk test represents a valid alternative to the molecular investigation of MBL genes, making MBL detection possible in routine diagnostic laboratories.

@article{valenza_first_2010-1, abstract = {A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-{beta}-lactamase {(MBL)} production. Molecular analysis detected a {blaVIM-1} gene in the chromosome of one isolate and a {blaVIM-2} gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory {EDTA} combination disk test represents a valid alternative to the molecular investigation of {MBL} genes, making {MBL} detection possible in routine diagnostic laboratories.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Valenza, Giuseppe and Joseph, Biju and Elias, Johannes and Claus, Heike and Oesterlein, Anett and Engelhardt, Kathrin and Turnwald, Doris and Frosch, Matthias and {Abele-Horn}, Marianne and Schoen, Christoph}, biburl = {https://www.bibsonomy.org/bibtex/21cbd8bdf57dbe85e6b2fd1a68f872854/jelias}, doi = {10.1128/AAC.00080-10}, interhash = {2159a4cbbbef0bcf34224e6cdbe362aa}, intrahash = {1cbd8bdf57dbe85e6b2fd1a68f872854}, journal = {Antimicrob. Agents Chemother.}, keywords = {imported}, month = aug, number = 8, pages = {3493--3497}, timestamp = {2011-03-11T10:06:29.000+0100}, title = {First Survey of {Metallo-{beta}-Lactamases} in Clinical Isolates of Pseudomonas aeruginosa in a German University Hospital}, url = {http://aac.asm.org/cgi/content/abstract/54/8/3493}, volume = 54, year = 2010 }