%0 Journal Article %1 little_strand_1999 %A Little, M C %A Andrews, J %A Moore, R %A Bustos, S %A Jones, L %A Embres, C %A Durmowicz, G %A Harris, J %A Berger, D %A Yanson, K %A Rostkowski, C %A Yursis, D %A Price, J %A Fort, T %A Walters, A %A Collis, M %A Llorin, O %A Wood, J %A Failing, F %A O'Keefe, C %A Scrivens, B %A Pope, B %A Hansen, T %A Marino, K %A Williams, K %D 1999 %J Clinical Chemistry %K Amplification, Bacterial, Chlamydia Diagnostic, Fluorescence, Gene Humans, Kits, Neisseria Probes, Reagent Sensitivity Specificity and gonorrhoeae, trachomatis, {DNA,} {DNA} %N 6 Pt 1 %P 777--784 %T Strand displacement amplification and homogeneous real-time detection incorporated in a second-generation DNA probe system, BDProbeTecET %U http://www.ncbi.nlm.nih.gov/pubmed/10351985 %V 45 %X BACKGROUND: Amplified DNA probes provide powerful tools for the detection of infectious diseases, cancer, and genetic diseases. Commercially available amplification systems suffer from low throughput and require decontamination schemes, significant hands-on time, and specially trained laboratory staff. Our objective was to develop a DNA probe system to overcome these limitations. METHODS: We developed a DNA probe system, the BDProbeTecTMET, based on simultaneous strand displacement amplification and real-time fluorescence detection. The system uses sealed microwells to minimize the release of amplicons to the environment. To avoid the need for specially trained labor, the system uses a simple workflow with predispensed reagent devices; a programmable, expandable-spacing pipettor; and the 96-microwell format. Amplification and detection time was 1 h, with potential throughput up to 564 patient results per shift. We tested 122 total patient specimens obtained from a family practice clinic with the BD ProbeTecET and the Abbott LCx(R) amplified system for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae. Results: Based on reportable results, the BDProbeTecET results for both organisms were 100\% sensitive and 100\% specific relative to the LCx. Conclusions: The BDProbeTecET is an easy-to-use, high-throughput, closed amplification system for the detection of nucleic acid from C. trachomatis and N. gonorrhoeae and other organisms.

@article{little_strand_1999, abstract = {{BACKGROUND:} Amplified {DNA} probes provide powerful tools for the detection of infectious diseases, cancer, and genetic diseases. Commercially available amplification systems suffer from low throughput and require decontamination schemes, significant hands-on time, and specially trained laboratory staff. Our objective was to develop a {DNA} probe system to overcome these limitations. {METHODS:} We developed a {DNA} probe system, the {BDProbeTecTMET,} based on simultaneous strand displacement amplification and real-time fluorescence detection. The system uses sealed microwells to minimize the release of amplicons to the environment. To avoid the need for specially trained labor, the system uses a simple workflow with predispensed reagent devices; a programmable, expandable-spacing pipettor; and the 96-microwell format. Amplification and detection time was 1 h, with potential throughput up to 564 patient results per shift. We tested 122 total patient specimens obtained from a family practice clinic with the {BD} {ProbeTecET} and the Abbott {LCx(R)} amplified system for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae. Results: Based on reportable results, the {BDProbeTecET} results for both organisms were 100\% sensitive and 100\% specific relative to the {LCx.} Conclusions: The {BDProbeTecET} is an easy-to-use, high-throughput, closed amplification system for the detection of nucleic acid from C. trachomatis and N. gonorrhoeae and other organisms.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Little, M C and Andrews, J and Moore, R and Bustos, S and Jones, L and Embres, C and Durmowicz, G and Harris, J and Berger, D and Yanson, K and Rostkowski, C and Yursis, D and Price, J and Fort, T and Walters, A and Collis, M and Llorin, O and Wood, J and Failing, F and {O'Keefe}, C and Scrivens, B and Pope, B and Hansen, T and Marino, K and Williams, K}, biburl = {https://www.bibsonomy.org/bibtex/208bf4bf729485ba355fbef6405fe286a/jelias}, interhash = {317693e3ea83ae8f4b7888d2ff47246f}, intrahash = {08bf4bf729485ba355fbef6405fe286a}, issn = {0009-9147}, journal = {Clinical Chemistry}, keywords = {Amplification, Bacterial, Chlamydia Diagnostic, Fluorescence, Gene Humans, Kits, Neisseria Probes, Reagent Sensitivity Specificity and gonorrhoeae, trachomatis, {DNA,} {DNA}}, month = jun, note = {{PMID:} 10351985}, number = {6 Pt 1}, pages = {777--784}, timestamp = {2011-03-11T10:05:41.000+0100}, title = {Strand displacement amplification and homogeneous real-time detection incorporated in a second-generation {DNA} probe system, {BDProbeTecET}}, url = {http://www.ncbi.nlm.nih.gov/pubmed/10351985}, volume = 45, year = 1999 }