The six out of seven gene over-expression constructs had a considerable impact on both ergosterol and β-amyrin production. In the case of β-amyrin formation the triple over-expression construct exhibited a nearly 500\% increase over the control strain making our metabolic engineering strategy the most successful design of triterpene microbial producers.
%0 Journal Article
%1 Madsen2011Linking
%A Madsen, Karina M.
%A Udatha, Gupta D.
%A Semba, Saori
%A Otero, Jose M.
%A Koetter, Peter
%A Nielsen, Jens
%A Ebizuka, Yutaka
%A Kushiro, Tetsuo
%A Panagiotou, Gianni
%D 2011
%J PloS one
%K metabolic-engineering yeast
%N 3
%R 10.1371/journal.pone.0014763
%T Linking Genotype and Phenotype of Saccharomyces cerevisiae Strains Reveals Metabolic Engineering Targets and Leads to Triterpene Hyper-Producers.
%U http://dx.doi.org/10.1371/journal.pone.0014763
%V 6
%X The six out of seven gene over-expression constructs had a considerable impact on both ergosterol and β-amyrin production. In the case of β-amyrin formation the triple over-expression construct exhibited a nearly 500\% increase over the control strain making our metabolic engineering strategy the most successful design of triterpene microbial producers.
@article{Madsen2011Linking,
abstract = {The six out of seven gene over-expression constructs had a considerable impact on both ergosterol and β-amyrin production. In the case of β-amyrin formation the triple over-expression construct exhibited a nearly 500\% increase over the control strain making our metabolic engineering strategy the most successful design of triterpene microbial producers.},
added-at = {2018-12-02T16:09:07.000+0100},
author = {Madsen, Karina M. and Udatha, Gupta D. and Semba, Saori and Otero, Jose M. and Koetter, Peter and Nielsen, Jens and Ebizuka, Yutaka and Kushiro, Tetsuo and Panagiotou, Gianni},
biburl = {https://www.bibsonomy.org/bibtex/25ecbd5c5fc75797468a42807c8f3c4e0/karthikraman},
citeulike-article-id = {9228059},
citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.pone.0014763},
citeulike-linkout-1 = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060802/},
citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/21445244},
citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=21445244},
doi = {10.1371/journal.pone.0014763},
interhash = {473e51c4e91513b514682248dcfe260d},
intrahash = {5ecbd5c5fc75797468a42807c8f3c4e0},
issn = {1932-6203},
journal = {PloS one},
keywords = {metabolic-engineering yeast},
number = 3,
pmcid = {PMC3060802},
pmid = {21445244},
posted-at = {2011-04-29 10:17:21},
priority = {2},
timestamp = {2018-12-02T16:09:07.000+0100},
title = {Linking Genotype and Phenotype of Saccharomyces cerevisiae Strains Reveals Metabolic Engineering Targets and Leads to Triterpene {Hyper-Producers}.},
url = {http://dx.doi.org/10.1371/journal.pone.0014763},
volume = 6,
year = 2011
}