Potassium ions (K+) are required for plant growth and development, including cell division and cell elongation/expansion, which are mediated by the K+ transport system. In this study, we investigated the role of K+ in cell division using tobacco BY-2 protoplast cultures. Gene expression analysis revealed induction of the Shaker-like outward K+ channel gene, NTORK1, under cell-division conditions, whereas the inward K+ channel genes NKT1 and NtKC1 were induced under both cell-elongation and cell-division conditions. Repression of NTORK1 gene expression by expression of its antisense construct repressed cell division but accelerated cell elongation even under conditions promoting cell division. A decrease in the K+ content of cells and cellular osmotic pressure in dividing cells suggested that an increase in cell osmotic pressure by K+ uptake is not required for cell division. In contrast, K+ depletion, which reduced cell-division activity, decreased cytoplasmic pH as monitored using a fluorescent pH indicator, SNARF-1. Application of K+ or the cytoplasmic alkalizing reagent (NH4)(2)SO4 increased cytoplasmic pH and suppressed the reduction in cell-division activity. These results suggest that the K+ taken up into cells is used to regulate cytoplasmic pH during cell division.
%0 Journal Article
%1 RN1130
%A Sano, T.
%A Kutsuna, N.
%A Becker, D.
%A Hedrich, R.
%A Hasezawa, S.
%D 2009
%J Plant Journal
%K cell division myOwn
%N 1
%P 55-64
%R 10.1111/j.1365-313X.2008.03672.x
%T Outward-rectifying K
channel activities regulate cell elongation and cell division of tobacco BY-2 cells
%U /brokenurl#<Go to ISI>://WOS:000261962700005
%V 57
%X Potassium ions (K+) are required for plant growth and development, including cell division and cell elongation/expansion, which are mediated by the K+ transport system. In this study, we investigated the role of K+ in cell division using tobacco BY-2 protoplast cultures. Gene expression analysis revealed induction of the Shaker-like outward K+ channel gene, NTORK1, under cell-division conditions, whereas the inward K+ channel genes NKT1 and NtKC1 were induced under both cell-elongation and cell-division conditions. Repression of NTORK1 gene expression by expression of its antisense construct repressed cell division but accelerated cell elongation even under conditions promoting cell division. A decrease in the K+ content of cells and cellular osmotic pressure in dividing cells suggested that an increase in cell osmotic pressure by K+ uptake is not required for cell division. In contrast, K+ depletion, which reduced cell-division activity, decreased cytoplasmic pH as monitored using a fluorescent pH indicator, SNARF-1. Application of K+ or the cytoplasmic alkalizing reagent (NH4)(2)SO4 increased cytoplasmic pH and suppressed the reduction in cell-division activity. These results suggest that the K+ taken up into cells is used to regulate cytoplasmic pH during cell division.
@article{RN1130,
abstract = {Potassium ions (K+) are required for plant growth and development, including cell division and cell elongation/expansion, which are mediated by the K+ transport system. In this study, we investigated the role of K+ in cell division using tobacco BY-2 protoplast cultures. Gene expression analysis revealed induction of the Shaker-like outward K+ channel gene, NTORK1, under cell-division conditions, whereas the inward K+ channel genes NKT1 and NtKC1 were induced under both cell-elongation and cell-division conditions. Repression of NTORK1 gene expression by expression of its antisense construct repressed cell division but accelerated cell elongation even under conditions promoting cell division. A decrease in the K+ content of cells and cellular osmotic pressure in dividing cells suggested that an increase in cell osmotic pressure by K+ uptake is not required for cell division. In contrast, K+ depletion, which reduced cell-division activity, decreased cytoplasmic pH as monitored using a fluorescent pH indicator, SNARF-1. Application of K+ or the cytoplasmic alkalizing reagent (NH4)(2)SO4 increased cytoplasmic pH and suppressed the reduction in cell-division activity. These results suggest that the K+ taken up into cells is used to regulate cytoplasmic pH during cell division.},
added-at = {2024-02-14T14:38:32.000+0100},
author = {Sano, T. and Kutsuna, N. and Becker, D. and Hedrich, R. and Hasezawa, S.},
biburl = {https://www.bibsonomy.org/bibtex/2047f42ee0b2fbba6c316ce27566d15a3/rainerhedrich_2},
doi = {10.1111/j.1365-313X.2008.03672.x},
interhash = {58e9e1aadfd70ccd4f3aecd8d70ff319},
intrahash = {047f42ee0b2fbba6c316ce27566d15a3},
issn = {0960-7412},
journal = {Plant Journal},
keywords = {cell division myOwn},
note = {387oy
Times Cited:27
Cited References Count:52},
number = 1,
pages = {55-64},
timestamp = {2024-02-14T14:38:32.000+0100},
title = {Outward-rectifying K
channel activities regulate cell elongation and cell division of tobacco BY-2 cells},
type = {Journal Article},
url = {/brokenurl#<Go to ISI>://WOS:000261962700005},
volume = 57,
year = 2009
}