Engineered DNA-binding domains provide a powerful technology for numerous biomedical studies due to their ability to recognize specific DNA sequences. Zinc fingers (ZF) are one of the most common DNA-binding domains and have been extensively studied for a variety of applications, such as gene regulation, genome engineering and diagnostics. Another novel DNA-binding domain known as a transcriptional activator-like effector (TALE) has been more recently discovered, which has a previously undescribed DNA-binding mode. Due to their modular architecture and flexibility, TALEs have been rapidly developed into artificial gene targeting reagents. Here, we describe the methods used to design these DNA-binding proteins and their key applications in biomedical research.
Description
This article discusses engineering of synthetic zinc finger proteins to bind to DNA in order to study it. I used it to get a sense of what zinc fingers were, since I noticed some of the important genes highlighted by Teschendorff et al. were involved with them
%0 Journal Article
%1 kim2017engineering
%A Kim, Moon-Soo
%A Kini, Anu Ganesh
%D 2017
%J Molecules and Cells
%K 5 background biotechnology zincFingers
%N 8
%P 533-541
%R 10.14348/molcells.2017.0139
%T Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research
%U http://www.molcells.org/journal/view.html?doi=10.14348/molcells.2017.0139
%V 40
%X Engineered DNA-binding domains provide a powerful technology for numerous biomedical studies due to their ability to recognize specific DNA sequences. Zinc fingers (ZF) are one of the most common DNA-binding domains and have been extensively studied for a variety of applications, such as gene regulation, genome engineering and diagnostics. Another novel DNA-binding domain known as a transcriptional activator-like effector (TALE) has been more recently discovered, which has a previously undescribed DNA-binding mode. Due to their modular architecture and flexibility, TALEs have been rapidly developed into artificial gene targeting reagents. Here, we describe the methods used to design these DNA-binding proteins and their key applications in biomedical research.
@article{kim2017engineering,
abstract = {Engineered DNA-binding domains provide a powerful technology for numerous biomedical studies due to their ability to recognize specific DNA sequences. Zinc fingers (ZF) are one of the most common DNA-binding domains and have been extensively studied for a variety of applications, such as gene regulation, genome engineering and diagnostics. Another novel DNA-binding domain known as a transcriptional activator-like effector (TALE) has been more recently discovered, which has a previously undescribed DNA-binding mode. Due to their modular architecture and flexibility, TALEs have been rapidly developed into artificial gene targeting reagents. Here, we describe the methods used to design these DNA-binding proteins and their key applications in biomedical research.},
added-at = {2017-10-05T00:41:23.000+0200},
author = {Kim, Moon-Soo and Kini, Anu Ganesh},
biburl = {https://www.bibsonomy.org/bibtex/2150fd71edae98ab24722c7ad7488ce55/artheibault},
description = {This article discusses engineering of synthetic zinc finger proteins to bind to DNA in order to study it. I used it to get a sense of what zinc fingers were, since I noticed some of the important genes highlighted by Teschendorff et al. were involved with them},
doi = {10.14348/molcells.2017.0139},
interhash = {ea36a16d4830c3befe727cbab3b913fb},
intrahash = {150fd71edae98ab24722c7ad7488ce55},
journal = {Molecules and Cells},
keywords = {5 background biotechnology zincFingers},
month = aug,
number = 8,
pages = {533-541},
timestamp = {2017-10-25T21:58:45.000+0200},
title = {Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research},
url = {http://www.molcells.org/journal/view.html?doi=10.14348/molcells.2017.0139},
volume = 40,
year = 2017
}