%0 Journal Article %1 valenza_first_2010 %A Valenza, Giuseppe %A Joseph, Biju %A Elias, Johannes %A Claus, Heike %A Oesterlein, Anett %A Engelhardt, Kathrin %A Turnwald, Doris %A Frosch, Matthias %A Abele-Horn, Marianne %A Schoen, Christoph %D 2010 %J Antimicrobial Agents and Chemotherapy %K Agents, Bacterial, Ceftazidime, Drug Germany, Hospitals, Humans, Imipenem, Infections Microbial Pseudomonas Resistance, Sensitivity Tests, University, aeruginosa, je, {Anti-Bacterial} {beta-Lactamases}, %N 8 %P 3493--3497 %R 10.1128/AAC.00080-10 %T First survey of metallo-beta-lactamases in clinical isolates of Pseudomonas aeruginosa in a German university hospital %U http://www.ncbi.nlm.nih.gov/pubmed/20498315 %V 54 %X A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-beta-lactamase (MBL) production. Molecular analysis detected a blaVIM-1 gene in the chromosome of one isolate and a blaVIM-2 gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory EDTA combination disk test represents a valid alternative to the molecular investigation of MBL genes, making MBL detection possible in routine diagnostic laboratories.

@article{valenza_first_2010, abstract = {A total of 489 clinical isolates of Pseudomonas aeruginosa was investigated for metallo-beta-lactamase {(MBL)} production. Molecular analysis detected a {blaVIM-1} gene in the chromosome of one isolate and a {blaVIM-2} gene carried on the plasmid in seven isolates. Moreover, we showed that an initial screening by combined susceptibility testing of imipenem and ceftazidime followed by a confirmatory {EDTA} combination disk test represents a valid alternative to the molecular investigation of {MBL} genes, making {MBL} detection possible in routine diagnostic laboratories.}, added-at = {2011-06-24T11:21:47.000+0200}, author = {Valenza, Giuseppe and Joseph, Biju and Elias, Johannes and Claus, Heike and Oesterlein, Anett and Engelhardt, Kathrin and Turnwald, Doris and Frosch, Matthias and {Abele-Horn}, Marianne and Schoen, Christoph}, biburl = {https://www.bibsonomy.org/bibtex/2c953b82d8158a1aebfe1619188eafc1e/ag_vogel}, doi = {10.1128/AAC.00080-10}, interhash = {2159a4cbbbef0bcf34224e6cdbe362aa}, intrahash = {c953b82d8158a1aebfe1619188eafc1e}, issn = {1098-6596}, journal = {Antimicrobial Agents and Chemotherapy}, keywords = {Agents, Bacterial, Ceftazidime, Drug Germany, Hospitals, Humans, Imipenem, Infections Microbial Pseudomonas Resistance, Sensitivity Tests, University, aeruginosa, je, {Anti-Bacterial} {beta-Lactamases},}, month = aug, note = {{PMID:} 20498315}, number = 8, pages = {3493--3497}, timestamp = {2014-02-06T18:33:35.000+0100}, title = {First survey of metallo-beta-lactamases in clinical isolates of Pseudomonas aeruginosa in a German university hospital}, url = {http://www.ncbi.nlm.nih.gov/pubmed/20498315}, volume = 54, year = 2010 }