A simple time-resolved fluorometer is constructed with an all-solid-state-based, frequency-tripled, Q-switched, diode-pumped Nd:YLF laser as the excitation source. Signal processing is accomplished with a digital oscilloscope. Simplicity of operation and applicability to trace analysis and in time-resolved spectroscopy are demonstrated with this new instrument. The laser produces 2.5-ns pulses at 349 nm and is capable of kilohertz repetition rates. For every shot of the laser, the oscilloscope collects an entire fluorescence decay at a 1-ns digitizer resolution and can average these data at the maximum laser repetition rate. When one is operating at 1 kHz and signal averaging for one second, detection limits (S/N = 3) in the 10-100 pM region are obtained. Excited-state decays are collected for several enzymatic probes and quinine sulfate, providing lifetimes consistent with those obtained by established instruments.
%0 Journal Article
%1 Basile1993
%A Basile, Franco
%A Cardamone, Anita
%A Grinstead, Keith D.
%A Miller, Karen J.
%A Lytle, Fred E.
%A Caprara, Andrea
%A Clark, Chris D.
%A Heritier, Jean-Marc
%D 1993
%J Appl. Spectrosc.
%K fluorescence hardware lifetime
%N 2
%P 207-210
%R 10.1366/0003702934048325
%T Simple Time-Resolved Fluorometer Based on a Nanosecond Digital Oscilloscope and a Diode-Pumped, Solid-State Laser
%V 47
%X A simple time-resolved fluorometer is constructed with an all-solid-state-based, frequency-tripled, Q-switched, diode-pumped Nd:YLF laser as the excitation source. Signal processing is accomplished with a digital oscilloscope. Simplicity of operation and applicability to trace analysis and in time-resolved spectroscopy are demonstrated with this new instrument. The laser produces 2.5-ns pulses at 349 nm and is capable of kilohertz repetition rates. For every shot of the laser, the oscilloscope collects an entire fluorescence decay at a 1-ns digitizer resolution and can average these data at the maximum laser repetition rate. When one is operating at 1 kHz and signal averaging for one second, detection limits (S/N = 3) in the 10-100 pM region are obtained. Excited-state decays are collected for several enzymatic probes and quinine sulfate, providing lifetimes consistent with those obtained by established instruments.
@article{Basile1993,
abstract = {A simple time-resolved fluorometer is constructed with an all-solid-state-based, frequency-tripled, Q-switched, diode-pumped Nd:YLF laser as the excitation source. Signal processing is accomplished with a digital oscilloscope. Simplicity of operation and applicability to trace analysis and in time-resolved spectroscopy are demonstrated with this new instrument. The laser produces 2.5-ns pulses at 349 nm and is capable of kilohertz repetition rates. For every shot of the laser, the oscilloscope collects an entire fluorescence decay at a 1-ns digitizer resolution and can average these data at the maximum laser repetition rate. When one is operating at 1 kHz and signal averaging for one second, detection limits (S/N = 3) in the 10-100 pM region are obtained. Excited-state decays are collected for several enzymatic probes and quinine sulfate, providing lifetimes consistent with those obtained by established instruments.},
added-at = {2010-03-09T21:58:22.000+0100},
author = {Basile, Franco and Cardamone, Anita and Grinstead, Keith D. and Miller, Karen J. and Lytle, Fred E. and Caprara, Andrea and Clark, Chris D. and Heritier, Jean-Marc},
biburl = {https://www.bibsonomy.org/bibtex/22168330a2c08415860b4e4b5be4468da/afcallender},
doi = {10.1366/0003702934048325},
file = {Basile1993.pdf:indexed\\Basile1993.pdf:PDF},
interhash = {7a5b3f027d24b2d141c6a194a8185dc8},
intrahash = {2168330a2c08415860b4e4b5be4468da},
journal = {Appl. Spectrosc.},
keywords = {fluorescence hardware lifetime},
month = {February},
number = 2,
pages = {207-210},
timestamp = {2010-03-09T22:20:10.000+0100},
title = {Simple Time-Resolved Fluorometer Based on a Nanosecond Digital Oscilloscope and a Diode-Pumped, Solid-State Laser},
volume = 47,
year = 1993
}